牛肾上腺髓质蛋白激酶C的快速纯化及性质
Rapid purification and properties of protein kinase C from bovine adrenal medulla.
作者信息
Brocklehurst K W, Lee G, Pollard H B
出版信息
Biosci Rep. 1986 Aug;6(8):749-57. doi: 10.1007/BF01116543.
PMID:3814769
Abstract
Protein kinase C was purified from bovine adrenal medulla using a rapid procedure which resulted in an approximate 1500-fold increase in specific activity. The characteristics of the enzyme are reported and for the first time it is possible to compare the effects of TPA on secretion from intact and permeabilized cells with the effect of TPA on protein kinase C purified from the same secretory tissue.
摘要
采用一种快速方法从牛肾上腺髓质中纯化蛋白激酶C，该方法使比活性提高了约1500倍。报道了该酶的特性，并且首次能够比较佛波酯（TPA）对完整细胞和通透细胞分泌的影响与TPA对从同一分泌组织纯化的蛋白激酶C的影响。
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