[Prokaryotic expression and biological activities of the hemolysin BL subunit of a pathogenic of cattle origin].

belongs to Gram-positive bacteria, which is widely distributed in nature and shows certain pathogenicity. Different . strains carry different subsets of virulence factors, which directly determine the difference in their pathogenicity. It is therefore important to study the distribution of virulence factors and the biological activity of specific toxins for precise prevention and control of . infection. In this study, the hemolysin BL triayl was expressed, purified, and characterized. The results showed that the bovine pathogenic . hemolysin BL could be expressed and purified in the prokaryotic expression system, and the bovine pathogenic . hemolysin BL showed hemolysis, cytotoxicity, good immunogenicity and certain immune protection in mice. In this study, the recombinant expression of hemolysin BL triayl was achieved, and the biological activity of hemolysin BL of bovine pathogenic ceroid spore was investigated. This study may facilitate further investigating the pathogenic mechanism of . hemolysin BL and developing a detection method for bovine pathogenic . disease.