Department of Plastic and Reconstructive Surgery, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Republic of Korea.
Department of Chemical and Biological Engineering, Sookmyung Women's University, Seoul, Republic of Korea.
In Vivo. 2024 Jan-Feb;38(1):235-245. doi: 10.21873/invivo.13430.
BACKGROUND/AIM: Hypertrophic scars (HS) are an abnormal cutaneous condition of wound healing characterized by excessive fibrosis and disrupted collagen deposition. This study assessed the potential of a silicone patch embedded with chemically stable zirconium-based metal-organic frameworks (MOF)-808 structures to mitigate HS formation using a rabbit ear model.
A silicone patch was strategically engineered by incorporating Zr-MOF-808, a composite structure comprising metal ions and organic ligands. Structural integrity of the Zr-MOF-808 silicone patch was validated using scanning electron microscopy and X-ray diffraction analysis. The animals were divided into three groups: a control, no treatment group (Group 1), a silicone patch treatment group (Group 2), and a group treated with a 0.2% loaded Zr-MOF-808 silicone patch (Group 3). HS suppression effects were quantified using scar elevation index (SEI), dorsal skin thickness measurements, and myofibroblast protein expression.
Histopathological examination of post-treatment HS samples revealed substantial reductions in SEI (34.6%) and epidermal thickness (49.5%) in Group 3. Scar hyperplasia was significantly diminished by 53.5% (p<0.05), while collagen density declined by 15.7% in Group 3 compared to Group 1. Western blot analysis of protein markers, including TGF-β1, collagen-1, and α-SMA, exhibited diminished levels by 8.8%, 12%, and 21.3%, respectively, in Group 3, and substantially higher levels by 21.9%, 27%, and 39.9%, respectively, in Group 2. On the 35th day post-wound generation, Zr-MOF-808-treated models exhibited smoother, less conspicuous, and flatter scars.
Zr-MOF-808-loaded silicone patch reduced HS formation in rabbit ear models by inducing the proliferation and remodeling of the wound healing process.
背景/目的：增生性瘢痕（HS）是一种异常的皮肤愈合状态，其特征是过度纤维化和胶原沉积紊乱。本研究采用兔耳模型评估了一种嵌入化学稳定锆基金属有机骨架（MOF）-808 结构的硅酮贴剂在减轻 HS 形成方面的潜力。
通过结合金属离子和有机配体的复合结构 Zr-MOF-808，对硅酮贴剂进行了策略性设计。通过扫描电子显微镜和 X 射线衍射分析验证了 Zr-MOF-808 硅酮贴剂的结构完整性。将动物分为三组：对照组、无治疗组（第 1 组）、硅酮贴剂治疗组（第 2 组）和 0.2%负载 Zr-MOF-808 硅酮贴剂治疗组（第 3 组）。使用瘢痕抬高指数（SEI）、背部皮肤厚度测量和肌成纤维蛋白表达来量化 HS 抑制效果。
治疗后 HS 样本的组织病理学检查显示，第 3 组的 SEI（34.6%）和表皮厚度（49.5%）显著降低。瘢痕增生减少了 53.5%（p<0.05），而胶原蛋白密度与第 1 组相比下降了 15.7%。第 3 组的 TGF-β1、胶原蛋白-1 和 α-SMA 等蛋白标志物的 Western blot 分析显示，相应蛋白水平分别下降了 8.8%、12%和 21.3%，而第 2 组则分别上升了 21.9%、27%和 39.9%。在伤口生成后的第 35 天，Zr-MOF-808 治疗的模型表现出更光滑、不明显和平坦的瘢痕。
Zr-MOF-808 负载的硅酮贴剂通过诱导伤口愈合过程的增殖和重塑，减少了兔耳模型中的 HS 形成。
