Laboratorio de Fitoquímica, Unidad de Biología, Tecnología y Prototipos (UBIPRO), Facultad de Estudios Superiores Iztacala (FESI), Universidad Nacional Autónoma de México (UNAM), Av de los Barrios # 1, Tlalnepantla, Estado de México, 54010, México.
Facultad de Medicina Veterinaria y Zootecnia (FMVZ), UNAM, Departamento de Pareasitología, Ciudad de México, 04510, México.
Parasitol Res. 2023 Dec 27;123(1):71. doi: 10.1007/s00436-023-08052-6.
The objective of this work was to evaluate the effect of the ethyl acetate extract from A. ludoviciana (EALM) and artemisinin against adult parasites and eggs of F. hepatica. For the ovicidal assay, cell culture plates with 24 wells were used, and 90 to 110 F. hepatica eggs were placed in each well. The eggs were exposed to concentrations of 100, 200, 300, 400, and 500 mg/L EALM and incubated for 16 days. Additionally, triclabendazole (TCBZ) was used as a reference drug at concentrations of 10 and 50 mg, and the response of artemisinin at concentrations of 10 and 20 mg was simultaneously assessed. Adult flukes were exposed to concentrations of 125, 250, 375, and 500 mg/L EALM. The results of the ovicidal action of EALM on the eggs showed that concentrations greater than 300 mg/L were significant, with ovicidal percentages greater than 60% observed on day 16 of incubation (p < 0.05). The maximum efficiency of EALM on adult flukes was reached 72 h post-exposure at a concentration of 125 mg/L (p < 0.05).
本研究旨在评估 A. ludoviciana（EALM）的乙酸乙酯提取物和青蒿素对肝片形吸虫成虫和虫卵的作用。在杀卵试验中，使用 24 孔细胞培养板，每孔放置 90 到 110 个肝片形吸虫卵。将卵暴露于 100、200、300、400 和 500mg/L 的 EALM 浓度下，并孵育 16 天。此外，还使用三氯苯达唑（TCBZ）作为参考药物，浓度为 10 和 50mg，并同时评估青蒿素在 10 和 20mg 的浓度下的反应。将成虫暴露于 125、250、375 和 500mg/L 的 EALM 浓度下。EALM 对虫卵的杀卵作用结果表明，浓度大于 300mg/L 时具有显著效果，孵育 16 天后观察到的杀卵率大于 60%（p<0.05）。EALM 对成虫的最大效率在暴露后 72 小时达到 125mg/L 的浓度（p<0.05）。
