Cai Zicheng, Zhu Chun, Hu Anqi, Chen Guoqing
School of Science, Jiangnan University, Wuxi, 214122, China.
Jiangsu Provincial Research Center of Light Industrial optoelectronic engineering and Technology, Wuxi, 214122, China.
J Fluoresc. 2025 Feb;35(2):661-672. doi: 10.1007/s10895-023-03567-8. Epub 2023 Dec 27.
The present study proposes a new approach for detecting trace amounts of creatinine (Cre) through the utilization of a fluorescence sensor system consisting of nitrogen doped carbon dots (NCDs) and gold ions (Au). Yellow fluorescent carbon dots were prepared using a one-step hydrothermal method with o-phenylenediamine and isopropanol as raw materials. First, gold ions are reduced to gold nanoparticles (Au NPs), which bind to NCDs, resulting in electron transfer and fluorescence quenching of NCDs. After adding creatinine, Cre and Au NPs were preferentially combined to form non-fluorescent complexes, and the NCDs fluorescence was restored. The study achieved a detection limit of 1.06 × 10 M for Au and 9.29 × 10 M for creatinine, indicating a high level of sensitivity. The sensing system has also been successfully utilized for detecting Au in lake water and Cre in human urine, indicating its promising potential and practical applications in the areas of environmental monitoring and biosensing.
本研究提出了一种通过利用由氮掺杂碳点（NCDs）和金离子（Au）组成的荧光传感器系统来检测痕量肌酐（Cre）的新方法。以邻苯二胺和异丙醇为原料，采用一步水热法制备了黄色荧光碳点。首先，金离子被还原为金纳米颗粒（Au NPs），其与NCDs结合，导致电子转移和NCDs的荧光猝灭。加入肌酐后，Cre与Au NPs优先结合形成非荧光复合物，NCDs荧光恢复。该研究实现了对Au的检测限为1.06×10 M，对肌酐的检测限为9.29×10 M，表明具有很高的灵敏度。该传感系统还成功用于检测湖水中的Au和人尿中的Cre，表明其在环境监测和生物传感领域具有广阔的应用前景和实际应用价值。
