Liu Di, Zhang Ying-Qi, Yu Tian-Yuan, Han Si-Long, Xu Ya-Jing, Guan Qian, Wang Hou-Rong
School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing, 102401, China.
Department of Acupuncture-Moxibustion, Dongfang Hospital Affiliated to Beijing University of Chinese Medicine, Beijing, 100078, China.
Heliyon. 2023 Dec 8;10(1):e23313. doi: 10.1016/j.heliyon.2023.e23313. eCollection 2024 Jan 15.
To investigate the mechanism of the six-method massage antipyretic process (SMAP) and its influence on the body's metabolic state.
The random number table method was used to divide 24 New Zealand 2-month-old rabbits with qualified basal body temperature into a control group, model group and massage group ( = 8 per group). The model group and massage groups were injected with 0.5 μg/ml lipopolysaccharide (1 ml/kg) into the auricular vein, and the control group was injected with the same amount of normal saline at the same temperature. One hour after modelling, the massage group was given SMAP (opening , pushing , rubbing , rubbing , clearing the and pushing the spine). The change of anal temperature 5 h after moulding was recorded to clarify the antipyretic effect.
After modelling, the rectal temperature of the juvenile rabbits in the three groups increased. The rectal temperature of the model group was higher than that of the control group 5 h after modelling, and the rectal temperature of the massage group was lower than that of the model group ( < 0.05). The antipyretic mechanism is related to the regulation of the synthesis of phenylalanine, tyrosine and tryptophan, as well as the pentose phosphate pathway. Compared with the model group, the plasma interleukin (IL)-1, IL-6, interferon-gamma, toll-like receptor 4, nuclear factor κB, the mechanistic target of rapamycin complex 1, indoleamine 2,3-dioxygenase 1, aryl hydrocarbon receptor, liver aspartate transaminase (AST), alanine transaminase (ALT) and l-glutamate dehydrogenase (L-GLDH) expression in the massage group were significantly decreased ( < 0.05). Compared with the model group, the massage group had significantly reduced AST, ALT and L-GLDH expression in plasma ( < 0.05).
The mechanism of SMAP therapy is related to regulating the expression of peripheral inflammatory factors and metabolic pathways.
探讨六法推拿退热过程（SMAP）的机制及其对机体代谢状态的影响。
采用随机数字表法将24只基础体温合格的2月龄新西兰兔分为对照组、模型组和推拿组（每组8只）。模型组和推拿组经耳静脉注射0.5μg/ml脂多糖（1ml/kg），对照组在相同温度下注射等量生理盐水。造模后1小时，推拿组给予六法推拿（开天门、推坎宫、揉太阳、揉耳后高骨、清天河水、推脊柱）。记录造模后5小时的肛温变化以明确退热效果。
造模后，三组幼兔的直肠温度均升高。造模后5小时，模型组的直肠温度高于对照组，推拿组的直肠温度低于模型组（P<0.05）。退热机制与苯丙氨酸、酪氨酸和色氨酸的合成调节以及磷酸戊糖途径有关。与模型组相比，推拿组血浆白细胞介素（IL）-1、IL-6、干扰素-γ、Toll样受体4、核因子κB、雷帕霉素靶蛋白复合物1、吲哚胺2,3-双加氧酶1、芳烃受体、肝脏天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和L-谷氨酸脱氢酶（L-GLDH）的表达均显著降低（P<0.05）。与模型组相比，推拿组血浆中AST、ALT和L-GLDH的表达显著降低（P<0.05）。
六法推拿疗法的机制与调节外周炎症因子表达及代谢途径有关。
