Department of Bacteriology and Immunology, Beijing Chest Hospital, Capital Medical University/Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, China.
Department of Infectious Diseases, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China.
Front Cell Infect Microbiol. 2023 Dec 12;13:1324611. doi: 10.3389/fcimb.2023.1324611. eCollection 2023.
In the fight against tuberculosis, besides chemotherapy, the regulation of oxidative stress (OS) has also aroused people's interest in host-oriented therapy. However, there is limited research on the genes involved in reactive oxygen species (ROS) production and clearance in macrophages infected with (MTB). This study analyzes and explores this to provide a basis for exploring new targets for antituberculosis treatments.
We established a macrophage model infected with MTB, counted intracellular bacteria, and determined the ROS produced using flow cytometry. We conducted ribonucleic acid sequencing, screened differentially expressed genes through transcriptomic methods, and validated the expression of them through reverse transcription-quantitative polymerase chain reaction.
The ROS of macrophages increased with intracellular bacteria at 4 h after infection with MTB and reached its peak at 48 h, surpassing the uninfected macrophages ( < 0.05). A total of 1,613 differentially expressed genes were identified after infection with MTB, of which 458 were associated with ROS, with over 50% involved in the response of organelles and biological processes to stimuli. We analyzed and identified six genes. After macrophage infection with MTB, the expression of increased, whereas the expression of decreased ( < 0.05). The expression of and increased, whereas the expression of decreased ( < 0.05).
The ROS-related differentially expressed genes between MTB infected and uninfected macrophages may be related to some organelles and involved in various biological processes, molecular functions, and signaling pathways. Among them, , and may be related to ROS.
在与结核病的斗争中，除了化疗外，氧化应激（OS）的调节也引起了人们对宿主导向治疗的兴趣。然而，人们对感染 （MTB）的巨噬细胞中涉及活性氧（ROS）产生和清除的基因的研究有限。本研究分析和探讨了这一点，为探索新的抗结核治疗靶点提供了依据。
我们建立了感染 MTB 的巨噬细胞模型，通过流式细胞术计数细胞内细菌，并确定产生的 ROS。我们进行了核糖核酸测序，通过转录组学方法筛选差异表达基因，并通过反转录定量聚合酶链反应验证其表达。
MTB 感染后 4 小时巨噬细胞的 ROS 增加，在 48 小时达到峰值，超过未感染的巨噬细胞（<0.05）。MTB 感染后共鉴定出 1613 个差异表达基因，其中 458 个与 ROS 相关，超过 50%涉及细胞器和生物过程对刺激的反应。我们分析并鉴定了六个基因。MTB 感染巨噬细胞后， 表达增加， 表达减少（<0.05）。 表达增加， 表达减少（<0.05）。
MTB 感染和未感染巨噬细胞之间与 ROS 相关的差异表达基因可能与某些细胞器有关，并涉及各种生物过程、分子功能和信号通路。其中， 、 和 可能与 ROS 有关。
