Stem cell and Regenerative Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran.
Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Zygote. 2024 Feb;32(1):87-95. doi: 10.1017/S096719942300062X. Epub 2023 Dec 27.
Mouse testicular tissue is composed of seminiferous tubules and interstitial tissue. Mammalian spermatogenesis is divided into three stages: spermatocytogenesis (mitotic divisions) in which spermatogonial stem cells (SSCs) turn into spermatocytes, followed by two consecutive meiotic divisions in which spermatocytes form spermatids. Spermatids differentiate into spermatozoa during spermiogenesis. Various factors affect the process of spermatogenesis and the organization of cells in the testis. Any disorder in different stages of spermatogenesis will have negative effects on male fertility. The aim of the current study was to compare the and spermatogenesis processes before and after transplantation to azoospermic mice using ultrastructural techniques. In this study, mice were irradiated with single doses of 14 Gy Co radiation. SSCs isolated from neonatal mice were cultured for 1 week and were injected into the seminiferous tubule recipient's mice. Testicular cells of neonatal mice were cultured in the four groups on extracellular matrix-based 3D printing scaffolds. The transplanted testes (8 weeks after transplantation) and cultured testicular cells (after 3 weeks) were then processed for transmission electron microscopy studies. Our study's findings revealed that the morphology and ultrastructure of testicular cells after transplantation and culture are similar to those of spermatogenesis, indicating that spermatogenic cell nature is unaltered .
小鼠睾丸组织由生精小管和间质组织组成。哺乳动物的精子发生分为三个阶段：精原细胞发生（有丝分裂），精原干细胞（SSC）转化为精母细胞，然后是两次连续的减数分裂，精母细胞形成精细胞。精细胞在精子发生过程中分化为精子。各种因素会影响精子发生过程和睾丸细胞的组织。精子发生不同阶段的任何障碍都会对男性生育能力产生负面影响。本研究旨在使用超微结构技术比较移植前后无精子症小鼠的 和 精子发生过程。在这项研究中，用 14 Gy Co 射线单次照射小鼠。从新生小鼠中分离出的 SSC 培养 1 周，然后注入生精小管受者小鼠体内。将新生小鼠的睾丸细胞在基于细胞外基质的 3D 打印支架上的四个组中进行培养。然后对移植睾丸（移植后 8 周）和培养的睾丸细胞（培养 3 周后）进行透射电子显微镜研究。我们的研究结果表明，移植后和 培养后的睾丸细胞的形态和超微结构与 精子发生相似，这表明生精细胞的性质没有改变。
