Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
Laboratory of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Monoclon Antib Immunodiagn Immunother. 2023 Dec;42(6):209-215. doi: 10.1089/mab.2023.0020. Epub 2023 Dec 26.
Immunohistochemistry staining is an essential method in pathological diagnoses. Podoplanin (PDPN) is a specific maker of alveolar epithelium, lymphatic vessels, and glomeruli. In this study, we established a novel anti-giraffe PDPN (girPDPN) mAb, PMab-301, using the Cell-Based Immunization and Screening (CBIS) method. PMab-301 (mouse IgG, kappa) detected girPDPN in various applications, such as flow cytometry, western blot, and immunohistochemistry. PMab-301 specifically stained type-I alveolar cells using formalin-fixed paraffin-embedded giraffe lung tissues. Our findings suggest the potential usefulness of PMab-301 for the pathophysiological analyses of giraffe tissues.
免疫组织化学染色是病理诊断中的一种重要方法。Podoplanin（PDPN）是肺泡上皮、淋巴管和肾小球的特异性标志物。本研究采用基于细胞的免疫接种和筛选（CBIS）方法，建立了一种新型抗长颈鹿 PDPN（girPDPN）单克隆抗体 PMab-301。PMab-301（小鼠 IgG，kappa）在流式细胞术、western blot 和免疫组织化学等多种应用中检测到 girPDPN。PMab-301 特异性地用福尔马林固定石蜡包埋的长颈鹿肺组织染色 I 型肺泡细胞。我们的研究结果表明，PMab-301 可能对长颈鹿组织的病理生理分析有用。
