抗牛血小板反应蛋白-1单克隆抗体PMab-44在免疫组织化学中可检测山羊血小板反应蛋白-1 。
Anti-Bovine Podoplanin Monoclonal Antibody PMab-44 Detects Goat Podoplanin in Immunohistochemistry.
作者信息
Yamada Shinji, Kaneko Mika K, Furusawa Yoshikazu, Itai Shunsuke, Sano Masato, Nakamura Takuro, Yanaka Miyuki, Handa Saori, Hisamatsu Kayo, Nakamura Yoshimi, Koyanagi Masanori, Fukui Masato, Harada Hiroyuki, Kato Yukinari
机构信息
1 Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan.
2 New Industry Creation Hatchery Center, Tohoku University, Sendai, Miyagi, Japan.
出版信息
Monoclon Antib Immunodiagn Immunother. 2019 Apr;38(2):96-99. doi: 10.1089/mab.2018.0031. Epub 2018 Nov 20.
Podoplanin (PDPN) is expressed in type I alveolar cells, kidney podocytes, and lymphatic endothelial cells. We have characterized the PDPNs of various animal species using specific anti-PDPN monoclonal antibodies (mAbs). In this study, we investigated whether these anti-PDPN mAbs cross-react with goat PDPN (gPDPN). Flow cytometry demonstrated that the anti-bovine PDPN mAb PMab-44 (IgG, kappa) reacts with gPDPN, which is overexpressed in CHO-K1 cells. Using immunohistochemical analysis, type I alveolar cells of goat lung were strongly detected by PMab-44. These results indicate that PMab-44 is useful for investigating gPDPN.
血小板源性生长因子受体β（Podoplanin，PDPN）在I型肺泡细胞、肾足细胞和淋巴管内皮细胞中表达。我们使用特异性抗PDPN单克隆抗体（mAb）对多种动物物种的PDPN进行了表征。在本研究中，我们调查了这些抗PDPN mAb是否与山羊PDPN（gPDPN）发生交叉反应。流式细胞术表明，抗牛PDPN mAb PMab-44（IgG，κ）与在CHO-K1细胞中过表达的gPDPN发生反应。使用免疫组织化学分析，PMab-44强烈检测到山羊肺的I型肺泡细胞。这些结果表明PMab-44可用于研究gPDPN。
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