Yamada Shinji, Kaneko Mika K, Furusawa Yoshikazu, Itai Shunsuke, Sano Masato, Nakamura Takuro, Yanaka Miyuki, Handa Saori, Hisamatsu Kayo, Nakamura Yoshimi, Koyanagi Masanori, Fukui Masato, Harada Hiroyuki, Kato Yukinari
1 Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan.
2 New Industry Creation Hatchery Center, Tohoku University, Sendai, Miyagi, Japan.
Monoclon Antib Immunodiagn Immunother. 2019 Apr;38(2):96-99. doi: 10.1089/mab.2018.0031. Epub 2018 Nov 20.
Podoplanin (PDPN) is expressed in type I alveolar cells, kidney podocytes, and lymphatic endothelial cells. We have characterized the PDPNs of various animal species using specific anti-PDPN monoclonal antibodies (mAbs). In this study, we investigated whether these anti-PDPN mAbs cross-react with goat PDPN (gPDPN). Flow cytometry demonstrated that the anti-bovine PDPN mAb PMab-44 (IgG, kappa) reacts with gPDPN, which is overexpressed in CHO-K1 cells. Using immunohistochemical analysis, type I alveolar cells of goat lung were strongly detected by PMab-44. These results indicate that PMab-44 is useful for investigating gPDPN.
血小板源性生长因子受体β（Podoplanin，PDPN）在I型肺泡细胞、肾足细胞和淋巴管内皮细胞中表达。我们使用特异性抗PDPN单克隆抗体（mAb）对多种动物物种的PDPN进行了表征。在本研究中，我们调查了这些抗PDPN mAb是否与山羊PDPN（gPDPN）发生交叉反应。流式细胞术表明，抗牛PDPN mAb PMab-44（IgG，κ）与在CHO-K1细胞中过表达的gPDPN发生反应。使用免疫组织化学分析，PMab-44强烈检测到山羊肺的I型肺泡细胞。这些结果表明PMab-44可用于研究gPDPN。
