Zhao Feng, Zhou Wenjuan, Xin Ran, Miao Xin
Department of Breast Surgery, The Second Affiliated Hospital of Shandong First Medical University, Taian 271000, Shandong, China.
Mutagenesis. 2024 Mar 12;39(2):141-145. doi: 10.1093/mutage/gead038.
Breast cancer is a malignant tumor in the epithelial tissue of the breast gland. This study aimed to unveil the expression and clinical diagnostic value of lncRNA cervical cancer high-expressed 1 (CCHE1) in breast cancer.
CCHE1 expression in breast cancer tissues was evaluated by RT-qPCR. The relationship between the CCHE1 expression and clinicopathological features of breast cancer was analyzed with the chi-square test, and the survival of breast cancer patients was evaluated with the Kaplan-Meier method. The diagnostic value of CCHE1 expression for breast cancer was evaluated by using the receiver operating characteristics (ROC) curve. Breast cancer cell lines (SKBR3, T47D, BT474, and MCF-7) were cultured for detecting CCHE1 expression in the cells. MCF-7 cells were selected for the subsequent experiments, and the small interfering RNA of CCHE1 (si-CCHE1) and CCHE1 overexpression vector (pcDNA-CCHE1) were transfected into MCF-7 cells. The proliferation, migration, and invasive ability were assessed by CCK-8 and Transwell assays. The influence of CCHE1 on the growth of tumors was validated by nude mice xenograft assay.
CCHE1 was up-regulated in breast cancer tissues and breast cancer cells. The high expression level of CCHE1 in cancer tissues of breast cancer patients was correlated with larger tumor size, advanced TNM stage, Ki-67 status, and lymph node metastasis. The area under the ROC curve for CCHE1 in the diagnosis of breast cancer was 0.983 (95% CI: 0.966-1.000), with a sensitivity of 95.00% and a specificity of 91.70%. The 5-year survival rate was higher in patients with low CCHE1 expression than those with high CCHE1 expression. Furthermore, restrained CCHE1 impeded proliferation, invasion, and migration of MCF-7 cells, as well as tumor growth in mice.
Our study highlights that elevated expression of CCHE1 in breast cancer tissues, which is closely related to clinicopathologic features, has some clinical value in the diagnosis of the disease.
乳腺癌是乳腺上皮组织中的恶性肿瘤。本研究旨在揭示长链非编码RNA宫颈癌高表达1（CCHE1）在乳腺癌中的表达情况及临床诊断价值。
采用逆转录定量聚合酶链反应（RT-qPCR）评估乳腺癌组织中CCHE1的表达。用卡方检验分析CCHE1表达与乳腺癌临床病理特征之间的关系，并用Kaplan-Meier法评估乳腺癌患者的生存率。通过绘制受试者工作特征（ROC）曲线评估CCHE1表达对乳腺癌的诊断价值。培养乳腺癌细胞系（SKBR3、T47D、BT474和MCF-7）以检测细胞中CCHE1的表达。选择MCF-7细胞进行后续实验，将CCHE1的小干扰RNA（si-CCHE1）和CCHE1过表达载体（pcDNA-CCHE1）转染到MCF-7细胞中。通过CCK-8和Transwell实验评估细胞的增殖、迁移和侵袭能力。通过裸鼠异种移植实验验证CCHE1对肿瘤生长的影响。
CCHE1在乳腺癌组织和乳腺癌细胞中表达上调。乳腺癌患者癌组织中CCHE1的高表达水平与肿瘤较大、TNM分期较晚、Ki-67状态及淋巴结转移相关。CCHE1诊断乳腺癌的ROC曲线下面积为0.983（95%CI：0.966-1.000），灵敏度为95.00%，特异性为91.70%。CCHE1低表达患者的5年生存率高于高表达患者。此外，抑制CCHE1可阻碍MCF-7细胞的增殖、侵袭和迁移以及小鼠肿瘤的生长。
我们的研究表明，乳腺癌组织中CCHE1表达升高与临床病理特征密切相关，对该疾病的诊断具有一定临床价值。
