Department of Molecular Genetics, Ahar Branch, Islamic Azad University, Ahar, Iran.
Mol Biol Rep. 2019 Oct;46(5):5419-5424. doi: 10.1007/s11033-019-04996-0. Epub 2019 Jul 30.
Genetic analysis has revealed that the lncRNAs acted in the critical pathways including cell growth, cell differentiation, and tumorigenesis. The purpose of the present survey was to evaluate the lncRNA-CCHE1 and lncRNA-HULC expression variations in subjects with cervical malignancy. In our case-control analysis planned on 100 formalin-fixed paraffin-embedded (FFPE) samples comprising 50 paraffin blocks of cervical cancerous and 50 paraffin blocks of non-tumors samples. We have calculated the lncRNA-CCHE1 and lncRNA-HULC gene expression alterations using a quantitative RT-PCR approach. Our results declared that the lncRNA-CCHE1 expression was considerably raised in tumorous matched to the non-tumorous samples (P = 0.001). Moreover, the lncRNA-HULC expression was not considerably modified between tumorous samples matched to the non-tumorous samples (P = 0.060). In extension, lncRNA-CCHE1 expression variation was correlated with the histological type (P = 0.001), tumor size (P = 0.001) and FIGO stage (P = 0.001). Our data advised that the lncRNA-CCHE1 gene may influence cervical malignancy progression, held as a prognostic marker and useful curative target. However, the character of lncRNA-HULC in carcinogenesis of cervical malignancy requires further analyses.
基因分析表明，lncRNAs 参与了包括细胞生长、细胞分化和肿瘤发生在内的关键途径。本研究旨在评估 lncRNA-CCHE1 和 lncRNA-HULC 在宫颈癌患者中的表达变化。在我们计划的 100 例福尔马林固定石蜡包埋（FFPE）样本的病例对照分析中，包括 50 例宫颈癌石蜡块和 50 例非肿瘤石蜡块。我们使用定量 RT-PCR 方法计算了 lncRNA-CCHE1 和 lncRNA-HULC 基因的表达变化。结果显示，lncRNA-CCHE1 在肿瘤组织中的表达明显高于非肿瘤组织（P=0.001）。此外，lncRNA-HULC 在肿瘤组织和非肿瘤组织之间的表达差异无统计学意义（P=0.060）。此外，lncRNA-CCHE1 的表达变化与组织学类型（P=0.001）、肿瘤大小（P=0.001）和 FIGO 分期（P=0.001）相关。我们的数据表明，lncRNA-CCHE1 基因可能影响宫颈癌的进展，可作为预后标志物和有价值的治疗靶点。然而，lncRNA-HULC 在宫颈癌发生中的作用需要进一步分析。
