Maeda T, Terada T, Tsutsumi Y
Br Poult Sci. 1986 Dec;27(4):695-702. doi: 10.1080/00071668608416929.
In order to investigate the factors causing crooked-necked spermatozoa (CNS) or those with abnormal acrosomes during freezing and thawing, fowl spermatozoa in NaCl or glucose solutions containing 92 ml glycerol/l were examined using a scanning electron microscope before and after freezing and thawing. The incidence of CNS in NaCl solution significantly increased after freezing and thawing, but not in glucose solution. The acrosomal damage caused by freezing and thawing was considerable in both solutions, and the incidence of damage in glucose solution was significantly higher than that in NaCl solution. In neither solution was there a significant difference between the incidence of acrosomal damage in CNS and in non-CNS. The ratios of incidences of abnormal acrosome after, versus before, freezing were higher in non-CNS than in CNS. It appears from these results that the factors during freezing and thawing which cause CNS may differ from those causing acrosomal damage.
为了研究在冷冻和解冻过程中导致弯颈精子（CNS）或顶体异常的因素，在含有92毫升甘油/升的氯化钠或葡萄糖溶液中，对家禽精子在冷冻和解冻前后进行扫描电子显微镜检查。冷冻和解冻后，氯化钠溶液中CNS的发生率显著增加，而葡萄糖溶液中则没有。两种溶液中冷冻和解冻引起的顶体损伤都相当严重，葡萄糖溶液中的损伤发生率显著高于氯化钠溶液。在两种溶液中，CNS和非CNS的顶体损伤发生率均无显著差异。非CNS冷冻后顶体异常发生率与冷冻前的比率高于CNS。从这些结果来看，冷冻和解冻过程中导致CNS的因素可能与导致顶体损伤的因素不同。
