Maeda T, Terada T, Tsutsumi Y
Br Poult Sci. 1984 Jul;25(3):409-13. doi: 10.1080/00071668408454881.
Fresh Muscovy drake spermatozoa were examined using a scanning electron microscope (SEM). The average lengths of the segments were: acrosome 1.8 micron, nucleus 10.9 micron, midpiece 3.6 micron and flagellum (exclusive of midpiece) 71 micron. Under the light microscope, the incidence of abnormal spermatozoa in Muscovy semen subjected to freezing and thawing (almost all with crooked necks) was about 5% higher than that in diluted unfrozen semen. In thawed semen, various abnormalities of the acrosome were observed under the SEM. It seemed that the most radical change was the complete separation of the acrosome from the apical part of the nucleus. The incidence of abnormal acrosomes was increased more than 20% by freezing and thawing. These results suggest that low fertility in thawed semen may be related to increases in the proportion of spermatozoa with crooked necks and acrosomal damage.
使用扫描电子显微镜（SEM）对新鲜的番鸭公鸭精子进行了检查。各部分的平均长度分别为：顶体1.8微米，细胞核10.9微米，中段3.6微米，鞭毛（不包括中段）71微米。在光学显微镜下，冷冻解冻后的番鸭精液中异常精子（几乎都是颈部弯曲）的发生率比稀释的未冷冻精液高约5%。在解冻精液中，通过扫描电子显微镜观察到顶体出现各种异常。似乎最根本的变化是顶体与细胞核顶端部分完全分离。冷冻解冻使异常顶体的发生率增加了20%以上。这些结果表明，解冻精液中低生育力可能与颈部弯曲精子比例增加和顶体损伤有关。
