Department of Hepatobiliary Surgery, Ningxia Medical University General Hospital, Yinchuan 750004, China.
Department of Medical Immunology and Pathogen Biology, Ningxia Medical University, Yinchuan 750004, China.
Acta Biochim Biophys Sin (Shanghai). 2024 Mar 25;56(3):482-489. doi: 10.3724/abbs.2023282.
Alveolar echinococcosis (AE) is a zoonotic parasitic disease caused by infection with the larval stage of and a major challenge to human public health. Vaccines are the most effective way to prevent and control infectious diseases. We previously revealed that the recombinant protein P29 is a good vaccine candidate against . . However, the protective and immunological mechanism of rEg.P29 against . remain unclear. In this study, CD4 T cell-deficient mice are transferred with spleen CD4 T cells isolated from wild-type mice and subjected to rEg.P29 immunization, and then these immunized mice are infected with . . The cyst inhibition rate is calculated by weighing the body and cyst weights. The level of antibody is detected by ELISA. Flow cytometry is used to detect the level of IFN-γ production by CD4 T and CD8 T cells. The cytokines in culture supernatant are detected by ELISA. The expressions of CD44 and CD62L on memory T cells are determined by flow cytometry. The results show the cyst inhibition rate is 41.52% after adoptive transfer of CD4 T cells. Furthermore, the levels of IgG, IgM, IgA and IgE in serum are significantly increased compared with those in the PBS group. The IFN-γ-secretion by CD8 T cells and the level of IFN-γ in culture supernatant are obviously increased; and the number of CD4 T cells is increased, but the number of IFN-γ producing CD4 T cells has no significant difference compared with PBS group. In addition, the number of CD44 CD62L CD8 memory T cells in the spleen is significantly increased, while the number of CD44 CD62L CD8 memory T cells is not significantly altered. Collectively, rEg.P29 can alleviate . infection by inducing humoral immune responses and CD8 T cell responses.
泡型包虫病（AE）是一种由幼虫阶段的 感染引起的人畜共患寄生虫病，是人类公共卫生的主要挑战。疫苗是预防和控制传染病最有效的方法。我们之前已经揭示， 重组蛋白 P29 是一种针对 的良好疫苗候选物。然而，rEg.P29 对 的保护和免疫机制仍不清楚。在这项研究中，将来自野生型小鼠的脾 CD4 T 细胞转移到 CD4 T 细胞缺陷型小鼠体内，并对其进行 rEg.P29 免疫接种，然后用 感染这些免疫接种的小鼠。通过称重体重和囊包重量来计算囊包抑制率。通过 ELISA 检测抗体水平。通过流式细胞术检测 CD4 T 和 CD8 T 细胞产生 IFN-γ的水平。通过 ELISA 检测培养上清液中的细胞因子。通过流式细胞术确定记忆 T 细胞上 CD44 和 CD62L 的表达。结果显示，CD4 T 细胞转移后囊包抑制率为 41.52%。此外，与 PBS 组相比，血清中 IgG、IgM、IgA 和 IgE 的水平显著增加。CD8 T 细胞分泌 IFN-γ和培养上清液中 IFN-γ的水平明显增加；CD4 T 细胞数量增加，但 IFN-γ产生的 CD4 T 细胞数量与 PBS 组相比无显著差异。此外，脾中 CD44 CD62L CD8 记忆 T 细胞的数量明显增加，而 CD44 CD62L CD8 记忆 T 细胞的数量无明显变化。综上所述，rEg.P29 可以通过诱导体液免疫反应和 CD8 T 细胞反应来缓解 感染。
