重组Eg.P29对绵羊细粒棘球绦虫的免疫保护作用
Immunoprotection of recombinant Eg.P29 against Echinococcus granulosus in sheep.
作者信息
Wang Hao, Li Zihua, Gao Fu, Zhao Jiaqing, Zhu Mingxing, He Xin, Niu Nan, Zhao Wei
机构信息
Department of Pathogenic Biology and Medical Immunology, Ningxia Medical University, Hui Autonomous Region, Yinchuan, Ningxia, 750004, China.
Key Laboratory of Hydatid Disease, Ningxia Medical University & Ningxia Institute of Medicine, Hui Autonomous Region, Yinchuan, Ningxia, 750004, China.
出版信息
Vet Res Commun. 2016 Jun;40(2):73-9. doi: 10.1007/s11259-016-9656-7. Epub 2016 Apr 19.
OBJECTIVE
This study aims to investigate the immunoprotection of recombinant Eg.P29 (rEg.P29) vaccine and analyze the underlying mechanism in sheep.
METHODS
Three groups of male sheep were immunized subcutaneously with rEg.P29 and PBS, Freund's complete adjuvant as controls, respectively. After prime-boost vaccination, the sheep were challenged with encapsulated Echinococcus granulosus eggs. The percentage of protection in sheep was determined 36 weeks after the infection. Humoral immune response was analyzed for specific IgG, IgG1, IgG2, IgM and IgE levels. Moreover, cytokines including interferon (IFN)-γ, interleukin (IL)-2, IL-4,and IL-10 were also evaluated.
RESULTS
Immunization with rEg.P29 induced protective immune responses up to 94.5 %, compared with immunoadjuvant group. The levels of specific IgG, IgG1, IgG2, and IgE as well as IFN-γ, IL-2, and IL-4 significantly increased after two immunizations (P < 0.05); however, the levels of IgM and IL-10 did not show difference.
CONCLUSION
rEg.P29 showed Immunoprotection and induced Th1 and Th2 immune responses; hence, rEg.P29 is a potential vaccine for E. granulosus infection.
目的
本研究旨在探讨重组Eg.P29（rEg.P29）疫苗在绵羊中的免疫保护作用，并分析其潜在机制。
方法
将三组雄性绵羊分别皮下注射rEg.P29、PBS和弗氏完全佐剂作为对照。在进行初次加强免疫接种后，用包囊型细粒棘球绦虫卵对绵羊进行攻击。在感染后36周测定绵羊的保护率。分析体液免疫反应中特异性IgG、IgG1、IgG2、IgM和IgE的水平。此外，还评估了包括干扰素（IFN）-γ、白细胞介素（IL）-2、IL-4和IL-10在内的细胞因子。
结果
与免疫佐剂组相比，用rEg.P29免疫诱导的保护免疫反应高达94.5%。两次免疫后，特异性IgG、IgG1、IgG2和IgE以及IFN-γ、IL-2和IL-4的水平显著升高（P < 0.05）；然而，IgM和IL-10的水平没有差异。
结论
rEg.P29具有免疫保护作用，并诱导Th1和Th2免疫反应；因此，rEg.P29是一种潜在的预防细粒棘球绦虫感染的疫苗。
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