Steele J G, Rupniak H T, Rowlatt C
Cancer Biochem Biophys. 1986 Dec;9(1):31-40.
The major cell surface glycoprotein components of four new cell lines derived from human squamous cell carcinomas of the head and neck (TR126, TR131, TR138, TR146, Rupniak, H. T. et al., JNCI 75, 621-635, 1985) were identified by three complementary labelling methods. The profile of labelled glycoprotein components was very similar in the four cell lines, although quite large quantitative differences in individual bands were seen. Two galactoproteins, designated GPC-130 and GPC-80 (apparent molecular weight X 10(-3)) were labelled by galactose oxidase/NaB [3H]4 but in all four lines only GPC-130 was prominent. The cell surface galactose and N-Acetylgalactosaminyl residues of glycoproteins were quite highly sialylated, as the galactose oxidase/NaB [3H]4 reaction was increased by between 3- and 6-fold after neuraminidase treatment. The neuraminidase-galactose oxidase/NaB [3H]4 and NaIO4/NaB [3H]4 methods identified a complex profile of glycoprotein components, with very high molecular weight sialogalactoconjugates being prominent. The major sialoglycoproteins were GPC-205, GPC-175, GPC-155, GPC-90 and GPC-70 and in addition, GPC-130 and GPC-80 showed enhanced labelling. Lactoperoxidase catalyzed the iodination of a similar profile of high molecular weight glycoprotein components, with GPC-205 and GPC-175 being prominent in TR126, TR131 and TR146 but less evident in TR138. Overall, the profile of labelled glycoprotein components was similar to the pattern seen in the well differentiated transitional carcinoma lines RT112 and RT4 (Steele, J. G. et al., Biochim. Biophys. Acta. 732, 219-228, 1983).
通过三种互补的标记方法鉴定了源自人头部和颈部鳞状细胞癌的四个新细胞系（TR126、TR131、TR138、TR146，鲁普尼亚克，H.T.等人，《美国国家癌症研究所杂志》75，621 - 635，1985年）的主要细胞表面糖蛋白成分。尽管在各个条带中观察到相当大的数量差异，但在这四个细胞系中标记的糖蛋白成分谱非常相似。两种半乳糖蛋白，命名为GPC - 130和GPC - 80（表观分子量×10(-3)），通过半乳糖氧化酶/NaB[3H]4进行标记，但在所有四个细胞系中只有GPC - 130很突出。糖蛋白的细胞表面半乳糖和N - 乙酰半乳糖胺残基高度唾液酸化，因为在神经氨酸酶处理后，半乳糖氧化酶/NaB[3H]4反应增加了3至6倍。神经氨酸酶 - 半乳糖氧化酶/NaB[3H]4和高碘酸钠/NaB[3H]4方法鉴定出糖蛋白成分的复杂谱，其中非常高分子量的唾液酸乳糖结合物很突出。主要的唾液酸糖蛋白是GPC - 205、GPC - 175、GPC - 155、GPC - 90和GPC - 70，此外，GPC - 130和GPC - 80显示出增强的标记。乳过氧化物酶催化了类似的高分子量糖蛋白成分谱的碘化反应，其中GPC - 205和GPC - 175在TR126、TR131和TR146中很突出，但在TR138中不太明显。总体而言，标记的糖蛋白成分谱与在高分化移行癌细胞系RT112和RT4中看到的模式相似（斯蒂尔，J.G.等人，《生物化学与生物物理学报》732，219 - 228，1983年）。
