School of Mechanical Engineering, Yonsei University, Seoul, 03722, South Korea.
Department of Medical Engineering, College of Medicine, Yonsei University, Seoul, 03722, South Korea.
Biosens Bioelectron. 2024 Nov 15;264:116658. doi: 10.1016/j.bios.2024.116658. Epub 2024 Aug 10.
To facilitate rapid monitoring of airborne viruses, they must be collected with high efficiency and concentrated in a small volume of a liquid sample. In addition, the development of low-cost miniaturized samplers is essential for multipoint monitoring. Thus, in an attempt to fulfill these requirements, this study developed a microfluidic condensation bioaerosol sampler (MCBS). The developed sampler comprised two parts: a virus growth section and a virus droplet-to-liquid sample conversion section, each of which was fabricated on a chip using microfluidic technology. The condensation nucleus growth technique used in the virus growth section grew nanometer-sized airborne viruses into micro-sized droplets, making it possible to collection of viruses easier and with high efficiency. In addition, the virus droplet-to-liquid sample conversion section controlled the transport of droplets based on electrowetting technology. This enabled the collected airborne viruses to be concentrated in tens of microliters of the liquid sample. To evaluate the performance of both the sections, the virus dropletization, virus collection efficiency, and virus droplet-to-liquid sample conversion efficiency were evaluated through quantitative experiments. H1N1 and HCOV-229E viruses were used to conduct quantitative experiments on MCBS. We could obtain virus liquid samples with at 72.8- and 89.9-times higher concentration through 1:1 evaluation with a commercial sampler. Thus, the developed sampler facilitated efficient collection and concentration of airborne viruses in a compact, cost-effective manner. This is expected to facilitate rapid and accurate multipoint monitoring of viral aerosols.
为了便于快速监测空气中的病毒，必须高效率地采集并将其浓缩在少量液体样本中。此外，开发低成本的小型化采样器对于多点监测至关重要。因此，本研究旨在开发一种微流控冷凝生物气溶胶采样器（MCBS），以满足这些要求。所开发的采样器由两部分组成：病毒生长部分和病毒液滴-液体样品转换部分，它们均使用微流控技术在芯片上制造。病毒生长部分中使用的核凝结技术将纳米级的空气中的病毒生长成微米级的液滴，从而使病毒更容易且高效地被采集。此外，病毒液滴-液体样品转换部分基于电润湿技术控制液滴的传输。这使得收集到的空气中的病毒可以浓缩在数十微升的液体样本中。为了评估两个部分的性能，通过定量实验评估了病毒液滴化、病毒收集效率和病毒液滴-液体样品转换效率。使用 H1N1 和 HCOV-229E 病毒对 MCBS 进行了定量实验。通过与商用采样器进行 1:1 的评估，我们可以获得 72.8 倍和 89.9 倍的高浓度病毒液体样本。因此，所开发的采样器以紧凑且经济高效的方式促进了空气中病毒的高效采集和浓缩。这有望促进对病毒气溶胶的快速准确多点监测。
