State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Nagqu City Inspection and Testing Center, Nagqu City, Tibet Autonomous Region 852000, China.
Food Chem. 2024 May 15;440:138247. doi: 10.1016/j.foodchem.2023.138247. Epub 2023 Dec 23.
Ophiocordyceps sinensis (OS), known as "soft gold", played an important role in local economic development. OS from different producing areas was difficult to be discriminated by the appearance. Nagqu OS, a distinguished and safeguarded geographical indication product, commands a premium price in market. The real claim of OS geographical origins is urgently required. Here, 81 OS samples were collected from Tibetan Plateau in China to explore markers for tracing origins. OS from Xigazê can be distinguished by dark color of head of caterpillar. Then 57 samples, a fully representative training-sample set, were used to set up OPLS-DA models by nontargeted metabolomics from UPLC-QTOF-MS. Certain markers were successfully identified and validation using 21 blind test samples confirmed that the markers can trace the geographical origin of OS, especially Nagqu samples. It was affirmed that UPLC-QTOF-MS-based untargeted metabolomics coupled with OPLS-DA was a reliable strategy to trace the geographical origins of OS.
冬虫夏草（OS），被称为“软黄金”，在中国的一些地区扮演着重要的经济发展角色。由于不同产地的冬虫夏草在外观上难以区分，因此，被认定为地理标志保护产品的那曲冬虫夏草在市场上的价格更高。然而，真正需要的是确定冬虫夏草的产地来源。在这里，我们从中国青藏高原收集了 81 个冬虫夏草样本，以探索可用于追溯产地的标记物。来自比如县的冬虫夏草头部颜色较深，可通过颜色来区分。然后，我们使用非靶向代谢组学方法（UPLC-QTOF-MS），从 57 个具有代表性的样本中建立了 OPLS-DA 模型。通过验证，成功地鉴定出了一些标记物，21 个盲测样本的验证结果表明，这些标记物可以追踪冬虫夏草的产地，特别是那曲地区的冬虫夏草。这也证明了基于 UPLC-QTOF-MS 的非靶向代谢组学与 OPLS-DA 相结合是一种可靠的追溯冬虫夏草产地的策略。
