Institute of Materia Medical, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, Shandong, China.
School of Clinical and Basic Medical Sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Mar 15;309:123794. doi: 10.1016/j.saa.2023.123794. Epub 2023 Dec 20.
Nitrites are ubiquitous in food and pose a serious threat to human health. Therefore, the rapid and accurate determination of nitrite ion concentration in food is a prerequisite for eliminating the damage of nitrites. In this study, a robust, rapid, and sensitive method is proposed for nitrite detection in pickled food, in which Au@Ag nanoparticles are used as a reliable surface-enhanced Raman spectroscopy (SERS) substrate taking advantage of the high enhancement effect of silver and the good stability of gold. Nitrites were anchored to the surface of the SERS substrate by bridging with 4-aminophenylthiophenol (PATP). With Raman scattering cross-section amplification and internal calibration by PATP, a satisfactory linear relationship (R = 0.987) was established for nitrite detection in the concentration range of 5.00-100.00 μM, and the limit of detection (LOD) was 0.17 μM. This SERS-based method demonstrated high selectivity, good precision (RSD < 7.00 %), and satisfying recovery rates (101.42-107.35 %) in real samples, thus improving the determination method for nitrites. Therefore, this method has application potential in food safety and supervision.
亚硝酸盐在食品中无处不在，对人类健康构成严重威胁。因此，快速准确地测定食品中亚硝酸盐离子的浓度是消除亚硝酸盐危害的前提。本研究提出了一种用于检测腌制食品中亚硝酸盐的稳健、快速和灵敏的方法，该方法利用银的高增强效应和金的良好稳定性，将金@银纳米粒子用作可靠的表面增强拉曼光谱（SERS）基底。亚硝酸盐通过与 4-氨基苯硫酚（PATP）桥接而被固定在 SERS 基底的表面。通过 PATP 的拉曼散射截面放大和内标，在 5.00-100.00 μM 的浓度范围内建立了满意的线性关系（R = 0.987），检测限（LOD）为 0.17 μM。该基于 SERS 的方法在实际样品中表现出高选择性、良好的精密度（RSD < 7.00%）和令人满意的回收率（101.42-107.35%），从而改进了亚硝酸盐的测定方法。因此，该方法在食品安全和监督方面具有应用潜力。
