School of Food Science and Technology, Ningxia University, 750021 Yinchuan, China.
Hubei Key Laboratory of Edible Wild Plants Conservation & Utilization, College of Life Sciences, Hubei Normal University, Huangshi 435002, China.
Int J Biol Macromol. 2024 Feb;258(Pt 2):128958. doi: 10.1016/j.ijbiomac.2023.128958. Epub 2023 Dec 26.
The level of polysaccharides in the mature Lycium barbarum fruit (LBF) cell wall depends on their metabolism, trafficking, and reassembly within the cell. In this study, we examined the composition, content, and ultrastructure of the cell wall polysaccharides of LBF during maturation, and further analyzed cell wall polysaccharide remodeling using isotope tagging with relative and absolute quantification (iTRAQ)-based proteomics. The results showed that the contents of cellulose and hemicellulose tended to increase in the pre-maturation stage and decrease in the later stage, while pectin level increased before fruit maturing. The differential expression of the 54 proteins involved in the metabolic pathways for glucose, fructose, galactose, galacturonic acid and arabinose was found to be responsible for these alterations. The work provides a biological framework for the reorganization of polysaccharides in the LBF cell wall, and supports the hypothesis that pectic polysaccharide glycosyl donors come from starch, cellulose, hemicellulose and isomorphic pectin.
成熟枸杞果实（LBF）细胞壁中多糖的水平取决于其在细胞内的代谢、运输和重新组装。在这项研究中，我们研究了 LBF 成熟过程中细胞壁多糖的组成、含量和超微结构，并进一步使用相对和绝对定量（iTRAQ）基于蛋白质组学的同位素标记分析了细胞壁多糖的重塑。结果表明，纤维素和半纤维素的含量在预成熟阶段趋于增加，在后期阶段减少，而果胶水平在果实成熟前增加。发现参与葡萄糖、果糖、半乳糖、半乳糖醛酸和阿拉伯糖代谢途径的 54 种蛋白质的差异表达是导致这些变化的原因。这项工作为 LBF 细胞壁中多糖的重新组合提供了一个生物学框架，并支持果胶多糖糖基供体来自淀粉、纤维素、半纤维素和同形果胶的假说。
