从宁夏枸杞果实中分离得到的一种多糖的生化分析及降血糖活性
Biochemical analysis and hypoglycemic activity of a polysaccharide isolated from the fruit of Lycium barbarum L.
作者信息
Tang Hua-Li, Chen Chen, Wang Shao-Kang, Sun Gui-Ju
机构信息
Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, and Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, PR China; School of Life Science and Engineering, Chongqing Three Gorges University, Chongqing 404000, PR China.
Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, and Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, PR China.
出版信息
Int J Biol Macromol. 2015;77:235-42. doi: 10.1016/j.ijbiomac.2015.03.026. Epub 2015 Mar 26.
Purification, characterization and hypoglycemic activities of polysaccharide from Lycium barbarum L. were investigated in this study. A water soluble polysaccharide (LBP) was obtained with ultrafiltration membranes separation, which was further purified by chromatography of DEAE cellulose column and Sephadex G-150 to get LBP3a and LBP3b. The high performance permeation chromatography (HPGPC) analysis showed that the average molecular weight (Mw) of LBP3b was 4.92kDa. Monosaccharide composition analysis revealed that the LBP3b was comprised of mannose, rhamnose, glucose, galactose and xylose with a molar ratio of 5.52:5.11:28.06:1.00:1.70. The preliminary structure features of LBP3b were investigated by UV, FT-IR, NMR and SEM. In vitro cell experiments showed that LBP3b had significantly inhibited the absorption of glucose in a dose-dependent manner. The study showed that LBP3b had potential use as an anti-diabetic agent.
本研究对枸杞多糖的纯化、表征及降血糖活性进行了研究。通过超滤膜分离得到一种水溶性多糖（LBP），进一步经DEAE纤维素柱层析和Sephadex G - 150纯化得到LBP3a和LBP3b。高效凝胶渗透色谱（HPGPC）分析表明，LBP3b的平均分子量（Mw）为4.92 kDa。单糖组成分析显示，LBP3b由甘露糖、鼠李糖、葡萄糖、半乳糖和木糖组成，摩尔比为5.52:5.11:28.06:1.00:1.70。通过紫外光谱（UV）、傅里叶变换红外光谱（FT - IR）、核磁共振（NMR）和扫描电子显微镜（SEM）对LBP3b的初步结构特征进行了研究。体外细胞实验表明，LBP3b能以剂量依赖的方式显著抑制葡萄糖的吸收。该研究表明，LBP3b具有作为抗糖尿病药物的潜在用途。
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