National Institute of Pharmaceutical Education and Research-Ahmedabad (NIPER-A), An Institute of National Importance, Government of India, Department of Pharmaceuticals, Ministry of Chemicals and Fertilizers, Palaj, Opp. Air force station, Gandhinagar 382355, Gujarat, India.
National Institute of Pharmaceutical Education and Research-Ahmedabad (NIPER-A), An Institute of National Importance, Government of India, Department of Pharmaceuticals, Ministry of Chemicals and Fertilizers, Palaj, Opp. Air force station, Gandhinagar 382355, Gujarat, India.
Int J Biol Macromol. 2024 Feb;258(Pt 2):129044. doi: 10.1016/j.ijbiomac.2023.129044. Epub 2023 Dec 27.
Cutaneous wounds are one of the pressing concerns for healthcare systems globally. With large amounts of water, conventional hydrogels encounter obstacles in effectively delivering small molecules and peptides for wound healing. The surplus water content challenges the stability and sustained release of small molecules and peptides, diminishing their therapeutic efficacy. Our pioneering smart powder bandage, fabricated through freeze-drying, ensures a water content of <1 % during storage. Upon contact with wound exudate, it forms hydrogel layers, thereby optimizing the delivery of peptides. Tailored for thermosensitive peptides such as EGF, this strategy surmounts the limitations of conventional hydrogels, providing a robust platform for efficacious therapeutic delivery in wound healing applications. Developing multifunctional wound dressings with antibacterial, anti-inflammatory, hemostatic, and healing properties is essential to promote wound healing. Therefore, the current investigation reports the development of multifunctional EGF@Silnanom SPB with the above-mentioned properties to promote wound healing using silver nanomix (Silnanom) and bioactive epidermal growth factors (EGF) as active therapeutics. The characterization of smart powder bandage (SPB) revealed that Silnanom were homogeneously dispersed in the entangled polymer network. The multifunctional smart powder bandage exhibited high bacterial inhibition rates against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), and rigorous hemocompatibility, cell compatibility, and in vivo studies also confirmed its biocompatibility. Furthermore, multifunctional EGF@Silnanom SPB effectively reduced pro-inflammatory markers, enhanced collagen deposition, promoted angiogenesis, and accelerated wound healing in a full-thickness mouse wound model through the sustained release of Silnanom and EGF. Additionally, the results of hemostasis analysis on the tail amputation mouse model confirmed the hemostasis properties of the EGF@Silnanom SPB. Overall, the multifunctional EGF@Silnanom SPB shows promising potential for skin wound repair, offering a potent and effective solution to the challenges posed by conventional wound dressings.
皮肤创伤是全球医疗保健系统面临的紧迫问题之一。由于大量的水，传统的水凝胶在有效输送小分子和肽类以促进伤口愈合方面遇到了障碍。过多的水分含量会影响小分子和肽类的稳定性和持续释放，从而降低其治疗效果。我们开创性的智能粉末绷带通过冷冻干燥制成，在储存过程中确保水分含量<1%。接触伤口渗出液后，它会形成水凝胶层，从而优化肽类的输送。针对表皮生长因子(EGF)等热敏肽类，该策略克服了传统水凝胶的局限性，为伤口愈合应用中的有效治疗药物输送提供了一个强大的平台。开发具有抗菌、抗炎、止血和愈合性能的多功能伤口敷料对于促进伤口愈合至关重要。因此，目前的研究报告了多功能 EGF@Silnanom SPB 的开发，该敷料具有上述特性，通过使用银纳米混合物（Silnanom）和生物活性表皮生长因子（EGF）作为活性治疗剂来促进伤口愈合。智能粉末绷带（SPB）的特性表明，Silnanom 均匀分散在缠结的聚合物网络中。多功能智能粉末绷带对大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）具有较高的抑菌率，且具有严格的血液相容性、细胞相容性和体内研究也证实了其生物相容性。此外，多功能 EGF@Silnanom SPB 通过持续释放 Silnanom 和 EGF，有效降低了促炎标志物，促进了胶原蛋白沉积，促进了血管生成，并加速了全厚度小鼠伤口模型中的伤口愈合。此外，尾巴切断小鼠模型的止血分析结果证实了 EGF@Silnanom SPB 的止血性能。总体而言，多功能 EGF@Silnanom SPB 具有皮肤伤口修复的巨大潜力，为传统伤口敷料面临的挑战提供了一种强大而有效的解决方案。
