Chowdhury K, Deutsch U, Gruss P
Cell. 1987 Mar 13;48(5):771-8. doi: 10.1016/0092-8674(87)90074-2.
Mouse genomic DNA contains multiple copies of sequences homologous to the Drosophila "Krüppel," a member of the "gap" class of developmental control genes of the fruit fly. The most interesting aspect of the homologous region is that, like Xenopus TFIIIA, it contains multiple finger-like folded domains capable of binding to nucleic acids. We have isolated six individual phages from a mouse genomic library on the basis of their DNA homology to Krüppel finger-coding probes, and describe here the DNA sequence and expression of two such clones containing finger-like structures. Upon differentiation of mouse teratocarcinoma cell line F9 with retinoic acid and cAMP, the expression of both genes was drastically reduced, and in one instance was undetectable. Each of the several other eukaryotic DNAs analyzed contained multiple copies of homologous genes with putative finger structures, indicating the presence of a finger-containing multigene family in higher organisms.
小鼠基因组DNA含有多个与果蝇“Krüppel”同源的序列拷贝，“Krüppel”是果蝇发育控制基因“间隙”类的成员。同源区域最有趣的方面是，与非洲爪蟾TFIIIA一样，它包含多个能够与核酸结合的手指状折叠结构域。我们基于与Krüppel手指编码探针的DNA同源性，从小鼠基因组文库中分离出六个单独的噬菌体，并在此描述两个含有手指状结构的此类克隆的DNA序列和表达情况。在用视黄酸和cAMP诱导小鼠畸胎瘤细胞系F9分化后，这两个基因的表达均大幅降低，在一个实例中甚至检测不到。分析的其他几种真核生物DNA中的每一种都含有多个具有推定手指结构的同源基因拷贝，表明高等生物中存在一个含手指结构的多基因家族。
