Chowdhury K, Dietrich S, Balling R, Guenet J L, Gruss P
Max-Planck-Institute for Biophysical Chemistry, Göttingen, FRG.
Nucleic Acids Res. 1989 Dec 25;17(24):10427-38. doi: 10.1093/nar/17.24.10427.
Zinc finger proteins (Zfp) are encoded by a large family of genes present in many organisms including yeast and human. Some of them are transcriptional activators and bind specifically to DNA by zinc mediated folded structures commonly known as zinc fingers. The Drosophila Krüppel (Kr) is a segmentation gene and encodes a zinc finger protein. Using a probe from the finger domain of Kr, we have isolated a structurally related gene Zfp-1 from the mouse. In this paper, we report the complete nucleotide sequence of two cDNA clones and the amino acid sequence deduced from them. The putative Zfp-1 protein contains in addition to 7 zinc fingers, two helix-turn-helix motifs. During murine embryogenesis, the Zfp-1 was found to express at a peak level in day 12 embryos. The ubiquitously expressed Zfp-1 gene is located in the 16q region on mouse chromosome 8, between the uvomorulin and the tyrosine amino transferase genes.
锌指蛋白（Zfp）由包括酵母和人类在内的许多生物体中存在的一大类基因编码。其中一些是转录激活因子，通过通常称为锌指的锌介导折叠结构特异性结合DNA。果蝇的Krüppel（Kr）是一个体节基因，编码一种锌指蛋白。我们使用来自Kr指状结构域的探针，从小鼠中分离出一个结构相关基因Zfp-1。在本文中，我们报告了两个cDNA克隆的完整核苷酸序列以及从中推导的氨基酸序列。推测的Zfp-1蛋白除了含有7个锌指外，还含有两个螺旋-转角-螺旋基序。在小鼠胚胎发育过程中，发现Zfp-1在第12天胚胎中表达达到峰值水平。普遍表达的Zfp-1基因位于小鼠8号染色体的16q区域内，在桥粒芯糖蛋白和酪氨酸氨基转移酶基因之间。
