Holthöfer H
Cell Differ. 1987 Jan;20(1):27-31. doi: 10.1016/0045-6039(87)90462-3.
Frozen sections of human fetal kidneys were studied for the appearance of vascular elements to the developing glomeruli using Ulex europaeus (UEA-I) lectin as a marker for endothelial cells. Chains of UEA-I-positive cells, seen to extend from larger vessels, could be observed among the uninduced cells of the nephrogenic mesenchyme. During the S-shaped body stage of nephrogenesis, the cleft of the comma-shaped cluster of epithelial cells was invaded by the UEA-I-positive cells, and during further glomerular development, the UEA-I-positive cells were seen to grow in number, prior to the appearance of visible lumina to the capillaries. The present results show, in addition to revealing the vascularization pattern of developing glomeruli, that endothelial cells obtain some of their characteristic glycoconjugates during early stages of differentiation. Thus, UEA-I seems to be a valuable tool to study in detail the vascularization of various developing human tissues.
利用欧洲荆豆（UEA-I）凝集素作为内皮细胞的标志物，对人类胎儿肾脏的冰冻切片进行研究，以观察发育中的肾小球的血管成分的出现情况。在肾发生间充质的未诱导细胞中，可以观察到从较大血管延伸出来的UEA-I阳性细胞链。在肾发生的S形期，UEA-I阳性细胞侵入上皮细胞逗号形簇的裂隙，在肾小球进一步发育过程中，在毛细血管出现可见管腔之前，UEA-I阳性细胞数量增加。目前的结果除了揭示发育中肾小球的血管形成模式外，还表明内皮细胞在分化早期获得了一些其特征性糖缀合物。因此，UEA-I似乎是详细研究各种发育中的人类组织血管形成的有价值工具。
