Huang Hongli, Pang Xianwu, Que Tengcheng, Chen Panyu, Li Shousheng, Wu Aiqiong, He Meihong, Qiu Hong, Hu Yanling
Clinical Biological Specimen Bank, Discipline Construction Office, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control, Nanning, Guangxi, China.
Front Microbiol. 2023 Dec 14;14:1309709. doi: 10.3389/fmicb.2023.1309709. eCollection 2023.
Understanding the gut microbiota and antibiotic resistance gene (ARG) profiles in non-human primates (NHPs) is crucial for evaluating their potential impact on human health and the environment.
In this study, we performed metagenomic analysis of 203 primate fecal samples, including nine NHP species and humans, to comprehensively characterize their gut microbiota and ARGs.
Our study reveals the prevailing phyla in primates as Firmicutes, Bacteroidetes, Euryarchaeota, and Proteobacteria. The captive NHPs exhibited higher ARG abundance compared to their wild counterparts, with tetracycline and beta-lactam resistance genes prevailing. Notably, ARG subtypes in Trachypithecus leucocephalus (T. leucocephalus) residing in karst limestone habitats displayed a more dispersed distribution compared to other species. Interestingly, ARG profiles of NHPs clustered based on geographic location and captivity status. Co-occurrence network analysis revealed intricate correlations between ARG subtypes and bacterial taxa. Procrustes analysis unveiled a significant correlation between ARGs and microbial phylogenetic community structure. Taxonomic composition analysis further highlighted differences in microbial abundance among NHPs and humans.
Our study underscores the impact of lifestyle and geographical location on NHP gut microbiota and ARGs, providing essential insights into the potential risks posed by NHPs to antibiotic resistance dissemination. This comprehensive analysis enhances our understanding of the interplay between NHPs and the gut resistome, offering a critical reference for future research on antibiotic resistance and host-microbe interactions.
了解非人类灵长类动物（NHPs）的肠道微生物群和抗生素抗性基因（ARG）谱对于评估它们对人类健康和环境的潜在影响至关重要。
在本研究中，我们对203份灵长类动物粪便样本进行了宏基因组分析，包括9种非人类灵长类动物和人类，以全面表征它们的肠道微生物群和抗生素抗性基因。
我们的研究揭示了灵长类动物中占主导地位的门类为厚壁菌门、拟杆菌门、广古菌门和变形菌门。圈养的非人类灵长类动物比野生同类表现出更高的抗生素抗性基因丰度，四环素和β-内酰胺抗性基因占主导。值得注意的是，生活在喀斯特石灰岩栖息地的白头叶猴（T. leucocephalus）的抗生素抗性基因亚型分布比其他物种更为分散。有趣的是，非人类灵长类动物的抗生素抗性基因谱根据地理位置和圈养状态聚类。共现网络分析揭示了抗生素抗性基因亚型与细菌分类群之间的复杂相关性。Procrustes分析揭示了抗生素抗性基因与微生物系统发育群落结构之间的显著相关性。分类组成分析进一步突出了非人类灵长类动物和人类之间微生物丰度的差异。
我们的研究强调了生活方式和地理位置对非人类灵长类动物肠道微生物群和抗生素抗性基因的影响，为非人类灵长类动物对抗生素抗性传播带来的潜在风险提供了重要见解。这种全面分析增强了我们对非人类灵长类动物与肠道抗性组之间相互作用的理解，为未来抗生素抗性和宿主-微生物相互作用的研究提供了关键参考。
