Achterberg V, Fricke H, Gercken G
Chem Phys Lipids. 1986 Oct-Nov;41(3-4):349-53. doi: 10.1016/0009-3084(86)90032-0.
Starting from biosynthetically prepared ethanolamine plasmalogen 14C-labelled in the O-alkenyl moiety, choline and dimethylethanolamine plasmalogen were prepared by transphosphatidylation utilizing phospholipase D from cabbage. Investigation of the time course of the reaction showed that transphosphatidylation was simultaneously accompanied by hydrolysis of both the substrate and the desired product, resulting in a maximum of product yield after 1-3 h under the reaction conditions investigated. Optimal reaction conditions gave yields of 40% and 62% (of total radioactivity) respectively for the purified choline and dimethylethanolamine derivatives.
从生物合成制备的、在O - 烯基部分标记有14C的乙醇胺缩醛磷脂开始，利用来自卷心菜的磷脂酶D通过转磷脂酰化反应制备胆碱和二甲基乙醇胺缩醛磷脂。对反应时间进程的研究表明，转磷脂酰化反应同时伴随着底物和所需产物的水解，在所研究的反应条件下，1 - 3小时后产物产率达到最高。优化的反应条件下，纯化的胆碱和二甲基乙醇胺衍生物的产率分别为40%和62%（基于总放射性）。
