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Synthesis of 6-phosphatidyl-L-ascorbic acid by phospholipase D.

作者信息

Nagao A, Ishida N, Terao J

机构信息

National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Ibaraki, Japan.

出版信息

Lipids. 1991 May;26(5):390-4. doi: 10.1007/BF02537205.

DOI:10.1007/BF02537205
PMID:1895887
Abstract

Phospholipase D (EC 3.1.4.4) of Streptomyces species was found to catalyze transphosphatidylation to L-ascorbic acid from phosphatidylcholine (PC) in a biphasic reaction system. The product was identified as 1,2-diacyl-sn-glycero-3-phospho-6'-L-ascorbic acid (PA-AsA) by mass spectrometry and nuclear magnetic resonance spectroscopy. The optimal pH of transphosphatidylation was 4.5 and the rate of PA-AsA formation increased as concentrations of L-ascorbic acid increased. The conversion of PC to PA-AsA was greater than 80%. PA-AsA was found to be more resistant to hydrolysis by phospholipase D than was PC.

摘要

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本文引用的文献

1
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Methods Enzymol. 1981;72:632-9. doi: 10.1016/s0076-6879(81)72055-x.
2
The formation of phosphatidylglycerol and other phospholipids by the transferase activity of phospholipase D.通过磷脂酶D的转移酶活性形成磷脂酰甘油和其他磷脂。
Biochem J. 1967 Jan;102(1):205-10. doi: 10.1042/bj1020205.
3
Transphosphatidylation by phospholipase D.磷脂酶D介导的转磷脂酰基作用
Lipids. 1994 Feb;29(2):83-9. doi: 10.1007/BF02537147.
J Biol Chem. 1967 Feb 10;242(3):477-84.
4
Synthetic I-acylglycerophospholipid analogues as lipid substrates. I. Studies on microsomal acylation and uptake into liver slices of I-acyl analogues containing various polar bases.
Biochim Biophys Acta. 1973 Jan 19;296(1):24-33. doi: 10.1016/0005-2760(73)90040-4.
5
Semi-synthetic approach for the preparation of homogeneous plasmenylethanolamine utilizing phospholipase D from Streptomyces chromofuscus.
J Lipid Res. 1985 May;26(5):629-33.
6
Conversion of radiolabelled ethanolamine plasmalogen into the dimethylethanolamine and choline analogue via transphosphatidylation by phospholipase D from cabbage.通过来自卷心菜的磷脂酶D的转磷脂酰基作用，将放射性标记的乙醇胺缩醛磷脂转化为二甲基乙醇胺和胆碱类似物。
Chem Phys Lipids. 1986 Oct-Nov;41(3-4):349-53. doi: 10.1016/0009-3084(86)90032-0.
7
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Chem Pharm Bull (Tokyo). 1987 Jan;35(1):447-9. doi: 10.1248/cpb.35.447.
8
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