NDRG2 通过 NLRP3/Caspase1 介导的焦亡促进晶状体上皮细胞衰老。
NDRG2 Promotes Lens Epithelial Cells Senescence via NLRP3/Caspase1-Mediated Pyroptosis.
机构信息
The Second Department of Ophthalmology, Cangzhou Central Hospital, Building 20, East District, Yunhe New Town, Cangzhou, 061000, Hebei Province, China.
出版信息
Appl Biochem Biotechnol. 2024 Aug;196(8):5435-5446. doi: 10.1007/s12010-023-04801-6. Epub 2023 Dec 30.
OBJECT
This study aims to investigate the molecular mechanism of NDRG2 (N-myc downstream-regulated gene 2) in the cell senescence of lens endothelial cells.
METHODS
Lens endothelial cells (SRA01/04) were irradiated with UVB at different times. Cell viability was measured by CCK-8 kit and cell cycle was detected by flow cytometry. Cell senescence was detected using SA-β-gal staining. Western blot was utilized to detect the expressions of p53, p21 and NDRG2. TUNEL staining and flow cytometry were used to detect apoptosis and pyroptosis.
RESULTS
UVB-irradiation significantly induces cell senescence and the expression of NDRG2, p53 and p21 in SRA01/04 cells was up-regulated. Down-regulation of NDRG2 inhibited UVB-induced cell senescence, significantly reversed pyroptosis and promoted cell proliferation. UVB-induced pyroptosis is closely related to caspase-1/NLRP3 axis.
CONCLUSION
Our study confirmed downregulation of NDRG2 significantly inhibited UVB radiation-induced cell senescence by regulating caspase-1/NLRP3-mediated pyroptosis.
目的
本研究旨在探讨 NDRG2（N-myc 下游调节基因 2）在晶状体内皮细胞衰老中的分子机制。
方法
用不同时间的 UVB 照射晶状体内皮细胞（SRA01/04）。通过 CCK-8 试剂盒测量细胞活力，通过流式细胞术检测细胞周期。用 SA-β-半乳糖苷染色检测细胞衰老。用 Western blot 检测 p53、p21 和 NDRG2 的表达。用 TUNEL 染色和流式细胞术检测细胞凋亡和焦亡。
结果
UVB 照射显著诱导细胞衰老，SRA01/04 细胞中 NDRG2、p53 和 p21 的表达上调。下调 NDRG2 抑制 UVB 诱导的细胞衰老，显著逆转焦亡并促进细胞增殖。UVB 诱导的焦亡与 caspase-1/NLRP3 轴密切相关。
结论
本研究证实下调 NDRG2 通过调节 caspase-1/NLRP3 介导的焦亡显著抑制 UVB 辐射诱导的细胞衰老。
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