Biochemistry and Environmental Toxicology Laboratory, Lab. # 103, School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.
Appl Biochem Biotechnol. 2023 Jan;195(1):172-195. doi: 10.1007/s12010-022-04145-7. Epub 2022 Sep 7.
Tupistra nutans Wall. ex Lindl. is a medicinal plant found in the Eastern Himalayan region. Besides being used as a folk medicine for pain and high blood sugar, its inflorescence is consumed as a vegetable. However, its medicinal properties have not been proven in vitro and in vivo till now. Therefore, in this study, we reported the phytochemicals present in the methanolic extract of Tupistra nutans Wall. ex Lindl. inflorescence (METNI) and its comparative effect in liver carcinoma HepG2 cells against non-cancerous murine fibroblast F111 cells. Phytochemical profiling by gas chromatography-mass spectrometry (GC-MS) analysis showed that METNI was rich in unsaturated fatty acids, vitamin E, and anticancer compounds like diosgenin, linoleic acid, and palmitoleic acid. METNI was found to have in vitro antioxidant property as determined by DPPH and pyrogallol methods, and UV protection property as investigated by fluorescence-based and spectrophotometric methods. MTT assay revealed METNI caused significantly more cell proliferation inhibition in HepG2 (IC = 138 µg/ml) compared to F111 (IC = 347 µg/ml) cells. Although in both HepG2 and F111 cells METNI showed significant antioxidant activity, it led to intracellular ROS generation and cell cycle alteration at higher exposure. The obtained results suggest that Tupistra nutans can be a promising application for anticancer drug and skin care product development, but can be harmful if overconsumed.
香榧（Tupistra nutans Wall. ex Lindl.）是一种生长在东喜马拉雅地区的药用植物。除了作为民间药物用于治疗疼痛和高血糖外，其花序也被当作蔬菜食用。然而，其药用特性至今尚未在体外和体内得到证实。因此，在这项研究中，我们报告了香榧花序甲醇提取物（METNI）中的植物化学物质，以及其在肝癌 HepG2 细胞和非癌细胞系 F111 小鼠成纤维细胞中的比较作用。气相色谱-质谱（GC-MS）分析的植物化学物质分析表明，METNI 富含不饱和脂肪酸、维生素 E 和具有抗癌作用的化合物，如薯蓣皂苷元、亚油酸和棕榈油酸。通过 DPPH 和焦性没食子酸法测定，发现 METNI 具有体外抗氧化特性，通过荧光法和分光光度法研究，发现其具有紫外线保护特性。MTT 试验表明，METNI 对 HepG2 细胞（IC=138µg/ml）的增殖抑制作用明显高于 F111 细胞（IC=347µg/ml）。尽管 METNI 在 HepG2 和 F111 细胞中均表现出显著的抗氧化活性，但在更高的暴露水平下，它会导致细胞内 ROS 生成和细胞周期改变。研究结果表明，香榧具有开发抗癌药物和皮肤护理产品的应用潜力，但如果过量食用可能会有害。
