Wang Xiao, Wang Xuechuan, Bai Zhongxue, Du Kaiqiang, Zhang Junli, Han Qingxin
College of Chemistry and Chemical Engineering, Shaanxi University of Science &Technology, Xi'an, 710021, PR China.
College of Chemistry and Chemical Engineering, Shaanxi University of Science &Technology, Xi'an, 710021, PR China; College of Bioresources Chemical and Materials Engineering, Shaanxi University of Science & Technology, Xi'an, 710021, PR China.
Talanta. 2024 Apr 1;270:125581. doi: 10.1016/j.talanta.2023.125581. Epub 2023 Dec 26.
The liver plays a crucial role in several important processes in the human body, including metabolism, detoxification, and immune function. When the liver experiences acute injury, it can cause significant harm and requires prompt detection. Traditional biomarkers lack specificity and cannot detect changes in real-time, making them unsuitable for monitoring pathological processes. Recent studies have shown that acute liver injury (ALI) is closely related to oxidative stress, with peroxynitrite (ONOO) being a vital byproduct of liver metabolism and become a critical biomarker for detecting liver damage. As a result, this research developed an activatable near-infrared fluorescent probe W-3a that can be used to detect endogenous ONOO in a mouse model of ALI induced by lipopolysaccharides (LPS). The probe has high selectivity and anti-interference ability, with a reaction time <10 min and a detection limit of 85 nM. It was successfully utilized in detecting endogenous ONOO in cells and live imaging of ALI mice.
肝脏在人体的几个重要过程中发挥着关键作用，包括新陈代谢、解毒和免疫功能。当肝脏发生急性损伤时，会造成严重损害，需要及时检测。传统生物标志物缺乏特异性，无法实时检测变化，因此不适用于监测病理过程。最近的研究表明，急性肝损伤（ALI）与氧化应激密切相关，过氧亚硝酸根（ONOO）是肝脏代谢的重要副产物，成为检测肝损伤的关键生物标志物。因此，本研究开发了一种可激活的近红外荧光探针W-3a，用于在脂多糖（LPS）诱导的ALI小鼠模型中检测内源性ONOO。该探针具有高选择性和抗干扰能力，反应时间<10分钟，检测限为85 nM。它已成功用于检测细胞中的内源性ONOO以及ALI小鼠的活体成像。
