An imidazo-pyridin derivative as fluorescent probe for the peroxynitrite detection in pulmonary permissive hypercapnia.

The physiological and pathological processes in lungs indicated that ONOO acted as an informative indicator for pulmonary functioning status with the corresponding dynamics, especially in permissive hypercapnia. Herein, for the peroxynitrite (ONOO) detection and imaging in living lung cells, an imidazo-pyridin derived fluorescent probe IDPD-ONOO was developed. IDPD-ONOO recognized ONOO to exhibit a corresponding fluorescence response at 490 nm with the excitation wavelength at 355 nm. The advantages of the developed probe included practical linear range (0-100 μM), high sensitivity (Limit of Detection 0.005 μM), rapid response (within 15 min), pH tolerance (7.0-9.0), high selectivity, and low cyto-toxicity. Furthermore, IDPD-ONOO achieved the pulmonary intracellular imaging of both the exogenous and endogenous ONOO level, which was meaningful for the research on the pulmonary physiological and pathological mechanism with optimized optical implements.