Animal Disease Diagnostic Laboratory, Purdue University, West Lafayette, IN, USA.
Center for Veterinary Medicine, Vet-LIRN, Food and Drug Administration, Laurel, MD, USA.
Res Vet Sci. 2024 Feb;167:105117. doi: 10.1016/j.rvsc.2023.105117. Epub 2023 Dec 22.
Manufacturers may intentionally or unintentionally incorporate ingredients not specified on the label of canned pet foods. Including any unacknowledged ingredients in a food product is considered food fraud or misbranding. Contamination of pet foods may occur in the processing of the foods, including potential cross-contamination in packaging facilities. Of the methods available to identify meat species in food products, Sanger sequencing and several next-generation sequencing methods are available, but there are limitations including the number of targets analyzed at a time and the method specificity. In this study, we developed a targeted next-generation sequencing panel to detect meat species in canned pet foods using Ion Torrent technology. The panel contains multiple primers targeting mitochondrial genes from as many as 27 animal species, of which 7 major animal species were validated. The meat species targets could be identified from samples spiked with as low as 0.01% w/w of the contaminating meat species in a vegetarian food matrix material. Targeted NGS in the current study enriches species-specific multiple target areas in the mitochondrial genome of the target material, which gives high accuracy in the sequencing results.
制造商可能会故意或无意地在罐装宠物食品的标签上加入未指定的成分。在食品中包含任何未被承认的成分都被认为是食品欺诈或标签错误。宠物食品的污染可能发生在食品加工过程中，包括包装设施中的潜在交叉污染。在可用于识别食品中肉类物种的方法中，Sanger 测序和几种下一代测序方法是可用的，但存在一些限制，包括一次分析的目标数量和方法特异性。在这项研究中，我们开发了一种靶向下一代测序面板，使用 Ion Torrent 技术检测罐装宠物食品中的肉类物种。该面板包含多个针对多达 27 种动物物种线粒体基因的引物，其中 7 种主要动物物种得到了验证。在素食食品基质材料中，即使仅污染了目标肉类物种的 0.01%w/w，也可以从添加的样本中识别出肉类物种的目标。本研究中的靶向 NGS 丰富了目标材料线粒体基因组中特定物种的多个目标区域，这使得测序结果具有很高的准确性。
