Aboghazleh Refat, Boyajian Silvia D, Atiyat Afnan, Udwan Manal, Al-Helalat Mimas, Al-Rashaideh Renad
Department of Basic Medical Sciences, Faculty of Medicine, Al-Balqa Applied University, The College of Medicine Building, Al-Salt 19117, Jordan.
Department of Medical Neuroscience, Faculty of Medicine and Brain Repair Center, Dalhousie University, Halifax, Canada.
MethodsX. 2023 Dec 7;12:102516. doi: 10.1016/j.mex.2023.102516. eCollection 2024 Jun.
The neuroscience is continuously expanding field, and conducting experiments serves as one of the most effective approaches to enhance and broad our understanding of this fascinating field. Most of the lab work in neuroscience involves the use of animal models such as rats and mice for experiments dedicated to monitoring cerebral changes. The study:•Introduces a practical method for brain extraction without perfusion with paraformaldehyde prioritizing brain integrity and avoiding damage.•Offers a detailed, step-by-step dissection guide for different brain regions, including the hippocampus, cerebral cortex, corpus striatum, thalamus, cerebellum, and medial prefrontal cortex, from rodent brains, accompanied by high-resolution images that provide anatomical clarity.•Presents enhanced reliability, precision, and detailed anatomical descriptions.: This study has introduced a reliable technique for brain extraction that eliminates the need for paraformaldehyde perfusion. Furthermore, a comprehensive methodology has been presented for extracting different brain regions from rodent brains.
神经科学是一个不断发展的领域，进行实验是增强和拓宽我们对这个迷人领域理解的最有效方法之一。神经科学中的大多数实验室工作都涉及使用大鼠和小鼠等动物模型进行实验，以监测大脑变化。该研究：
• 介绍了一种在不使用多聚甲醛灌注的情况下进行脑提取的实用方法，优先考虑脑的完整性并避免损伤。
• 提供了一份针对不同脑区（包括海马体、大脑皮层、纹状体、丘脑、小脑和内侧前额叶皮层）的详细分步解剖指南，取材于啮齿动物大脑，并配有提供解剖清晰度的高分辨率图像。
• 具有更高的可靠性、精度和详细的解剖描述。：本研究引入了一种可靠的脑提取技术，无需多聚甲醛灌注。此外，还提出了一种从啮齿动物大脑中提取不同脑区的综合方法。
