Laboratory of Modern Biotechnology, School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei 230036, China.
J Plant Physiol. 2024 Feb;293:154166. doi: 10.1016/j.jplph.2023.154166. Epub 2023 Dec 28.
When plants are entirely submerged, photosynthesis and respiration are severely restricted, affecting plant growth and potentially even causing plant death. The AP2/ERF superfamily has been widely reported to play a vital role in plant growth, development and resistance to biotic and abiotic stresses. However, no relevant studies exist on flooding stress in pecan. In this investigation, we observed that CiAP2/ERF65 positively modulated the hypoxia response during submergence, whereas CiAP2/ERF106 was sensitive to submergence. The levels of physiological and biochemical indicators, such as POD, CAT and among others, in CiAP2/ERF65-OE lines were significantly higher than those in wild-type Arabidopsis thaliana, indicating that the antioxidant capacity of CiAP2/ERF65-OE lines was enhanced under submergence. The RNA-seq results revealed that the maintenance of the expression levels of the antenna protein gene, different signaling pathways for regulation, as well as the storage and consumption of ATP, might account for the opposite phenotypes of CiAP2/ERF65 and CiAP2/ERF106. Furthermore, the expression of some stress-related genes was altered during submergence and reoxygenation. Overall, these findings enhance our understanding of submergence stress in pecan, providing important candidate genes for the molecular design and breeding of hypoxia resistant in plants.
当植物完全被淹没时，光合作用和呼吸作用会受到严重限制，从而影响植物的生长，甚至可能导致植物死亡。AP2/ERF 超家族已被广泛报道在植物的生长、发育和对生物及非生物胁迫的抗性中起着至关重要的作用。然而，关于山核桃对水淹胁迫的研究还很少。在这项研究中，我们观察到 CiAP2/ERF65 对淹水时的缺氧反应有正向调节作用，而 CiAP2/ERF106 对淹水敏感。CiAP2/ERF65-OE 系的 POD、CAT 等生理生化指标的水平明显高于野生型拟南芥，表明 CiAP2/ERF65-OE 系在淹水条件下的抗氧化能力增强。RNA-seq 结果表明，天线蛋白基因表达水平的维持、不同的调控信号通路以及 ATP 的储存和消耗，可能解释了 CiAP2/ERF65 和 CiAP2/ERF106 的表型相反的原因。此外，一些与胁迫相关的基因在淹水和再氧合过程中表达发生改变。总的来说，这些发现加深了我们对山核桃水淹胁迫的理解，为植物耐缺氧的分子设计和育种提供了重要的候选基因。
