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气相色谱-质谱分析健康阿拉比卡咖啡豆和感染黑曲霉 MH078571.1 的阿拉比卡咖啡豆中的脂肪酸。

Gas chromatography-mass spectrometry analysis of fatty acids in healthy and Aspergillus niger MH078571.1-infected Arabica coffee beans.

机构信息

Department of Biology, College of Science, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.

Basic and Applied Scientific Research Center (BASRC), Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.

出版信息

PLoS One. 2024 Jan 2;19(1):e0293369. doi: 10.1371/journal.pone.0293369. eCollection 2024.

Abstract

The organic composition of Arabica coffee beans, particularly fatty acids, significantly influences their overall quality. After measuring its composition of fatty acids, it contained a high percentage of saturated fatty acids (SFA), including caprylic, lauric, myristic, palmitic, margaric, fat, and orchid. Moreover, the sample contained unsaturated fatty acids (USFA), namely palmitoleic acid (C16:1), oleic acid (C18:1), linoleic acid (C18:2), and alpha-linoleic acid (C18:3). Coffee beans are susceptible to infection by fungi during storage, the development of which has adverse effects on the beans. The present study aimed to examine the impact of Aspergillus niger MH078571.1 infection on the diversity and abundance of fatty acids in green Arabica coffee beans. The impact of Aspergillus niger on the consumption of fatty acids in Arabica coffee beans was assessed. The findings of the study indicate that the duration of storage had a significant impact on the levels of fatty acids, specifically miristic (C14:0), margaric (C17:0), and stearic (C18:0), which increased as the storage period and temperature increased. Conversely, the percentage of oleic acid decreased under these conditions. This trend was observed across different storage temperatures (0, 8, and 25°C) in untreated coffee beans affected by fungal activity.

摘要

阿拉比卡咖啡豆的有机成分,特别是脂肪酸,对其整体质量有重大影响。在测量其脂肪酸组成后,发现其中含有高比例的饱和脂肪酸(SFA),包括辛酸、月桂酸、肉豆蔻酸、棕榈酸、十七烷酸、脂肪和兰花酸。此外,该样本还含有不饱和脂肪酸(USFA),即棕榈油酸(C16:1)、油酸(C18:1)、亚油酸(C18:2)和α-亚麻酸(C18:3)。咖啡豆在储存过程中容易受到真菌的感染,真菌的生长会对咖啡豆产生不利影响。本研究旨在研究黑曲霉 MH078571.1 感染对绿阿拉比卡咖啡豆中脂肪酸多样性和丰度的影响。评估了黑曲霉对阿拉比卡咖啡豆中脂肪酸消耗的影响。研究结果表明,储存时间对脂肪酸水平有显著影响,特别是豆蔻酸(C14:0)、十七烷酸(C17:0)和硬脂酸(C18:0),随着储存时间和温度的升高而增加。相反,在这些条件下,油酸的百分比下降。这种趋势在未受真菌活动影响的不同储存温度(0、8 和 25°C)下的咖啡豆中都观察到。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6459/10760658/883d7f925be4/pone.0293369.g001.jpg

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