Haffner H, Appel H, Holmes K C
Eur Biophys J. 1986;14(1):37-41. doi: 10.1007/BF00260401.
The Moessbauer method was applied to obtain information on a suggested hydrogen bond in tobacco mosaic virus (TMV), between the hydroxyl group of Tyr 139 and a carboxyl oxygen of Glu 22 in a neighbouring subunit. Spectra of 129I were taken of 3,5-di-iodo-L-tyrosine as a free amino acid and in situ in TMV. The increase of the pK value of 3,5-di-iodo-L-tyrosine by 0.8 units at position 139 in TMV compared to the free value is a strong argument in favour of the existence of a hydrogen bond via the relevant hydroxyl group. The reported study demonstrates the surprising sensitivity of the observable Moessbauer parameters to details of the electronic configuration in the neighbourhood of the probe nucleus.
采用穆斯堡尔方法来获取有关烟草花叶病毒（TMV）中一个推测的氢键的信息，该氢键存在于相邻亚基中Tyr 139的羟基与Glu 22的羧基氧之间。对作为游离氨基酸的3,5-二碘-L-酪氨酸以及TMV原位的129I光谱进行了测定。与游离状态相比，TMV中139位的3,5-二碘-L-酪氨酸的pK值增加了0.8个单位，这有力地证明了通过相关羟基存在氢键。所报道的研究表明，可观测的穆斯堡尔参数对探针原子核附近电子构型的细节具有惊人的敏感性。
