Dept. of Biotechnology, School of Life Sciences, Central University of Rajasthan, Ajmer, Bandersindri NH 8, Kishangarh Dist Ajmer (Rajasthan), India.
School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded, 431606, MS, India.
BMC Res Notes. 2024 Jan 2;17(1):2. doi: 10.1186/s13104-023-06661-z.
Candida albicans, a polymorphic yeast, is one of the most common, opportunistic fungal pathogens of humans. Among the different morphological forms, opaque form is one of the least-studied ones. This opaque phenotype is essential for mating and is also reported to be involved in colonizing the gastrointestinal tract. Considering the significance of the clinical and sexual reproduction of C. albicans, we have investigated the morphophysiological modulations in opaque form using a proteomic approach.
In the current investigation, we have used Micro-Liquid Chromatography-Mass Spectrometry (LC-MS/MS) analysis to create a protein profile for opaque-specific proteins. Whole-cell proteins from C. albicans (ATCC10231) cells that had been cultured for seven days on synthetic complete dextrose (SCD) medium in both as an opaque (test) and as a white (control) form cells were extracted, digested, and identified using LC-MS/MS. This information is meant to serve the scientific community and represents the proteome profile (SWATH Spectral Libraries) of C. albicans opaque form.
白色念珠菌是一种多态酵母，是人类最常见的机会性真菌病原体之一。在不同的形态形式中，不透明形式是研究最少的形式之一。这种不透明表型对于交配至关重要，据报道也参与了胃肠道的定植。考虑到白色念珠菌的临床和有性繁殖的重要性，我们使用蛋白质组学方法研究了不透明形式的形态生理调节。
在当前的研究中，我们使用微液相色谱-质谱联用（LC-MS/MS）分析技术为不透明特异性蛋白创建了蛋白质图谱。从白色念珠菌（ATCC10231）细胞中提取全细胞蛋白，这些细胞在合成完全葡萄糖（SCD）培养基中培养了七天，分别呈不透明（测试）和白色（对照）两种形式。使用 LC-MS/MS 对细胞进行消化和鉴定。这些信息旨在为科学界服务，代表了白色念珠菌不透明形式的蛋白质组图谱（SWATH 光谱文库）。
