Sedaghati Golnoosh, Feizbakhsh Masood, Esnaashari Nasim, Razavi Seyed Mohammad
Department of Orthodontics, School of Dentistry, Islamic Azad University, Isfahan (Khorasgan) Branch, Isfahan, Iran.
Craniofacial and Cleft Research Center, Isfahan University of Medical Science, Isfahan, Iran.
Dent Res J (Isfahan). 2023 Nov 27;20:118. eCollection 2023.
This study aimed to assess the effect of local administration of injectable platelet-rich fibrin (i-PRF) on root resorption during orthodontic tooth movement in dogs.
This animal study was conducted on 4 adult male mongrel dogs. The right and left maxillary first premolars of the dogs were extracted, and a Nickel-Titanium closed coil spring was used to connect the canine to the second premolar with 150 g load. Next, 0.5 cc of i-PRF was injected in one quadrant of the maxilla around the second premolar into the periodontal ligament. The other quadrant of the maxilla served as the control group and received saline injection. Injections were performed at 1, 21, and 42 days, and the dogs were sacrificed after 63 days. Histological sections were prepared and cementum resorption, secondary cementum formation, and number of cementoblasts and cementoclasts were compared between the two groups by the Friedman test, Wilcoxon test, and Mann-Whitney test (α = 0.05).
The mean percentage of cementum resorption (17.75% ± 5.56%) and secondary cementum formation (14.50% ± 6.65%), and the mean number of cementoblasts (10.25 ± 2.36) and cementoclasts (9.75 ± 4.71) were insignificantly higher in the i-PRF group than the corresponding values (13.75% ± 4.34%, 8.50% ± 2.88%, 7.75 ± 1.25, and 6.50 ± 3.10, respectively) in the control group ( > 0.05).
Administration of i-PRF insignificantly increased the percentage of cementum resorption, secondary cementum formation, number of cementoblasts, and number of cementoclasts.
本研究旨在评估局部注射富血小板纤维蛋白（i-PRF）对犬正畸牙齿移动过程中牙根吸收的影响。
本动物研究对4只成年雄性杂种犬进行。拔除犬的左右上颌第一前磨牙，使用镍钛闭合螺旋弹簧以150克的负荷将尖牙与第二前磨牙相连。接下来，在犬上颌的一个象限，于第二前磨牙周围的牙周膜内注射0.5毫升i-PRF。上颌的另一个象限作为对照组，注射生理盐水。在第1天、21天和42天进行注射，63天后处死犬。制备组织学切片，通过Friedman检验、Wilcoxon检验和Mann-Whitney检验（α = 0.05）比较两组的牙骨质吸收、继发性牙骨质形成以及成牙骨质细胞和成牙骨质破骨细胞的数量。
i-PRF组的牙骨质吸收平均百分比（17.75% ± 5.56%）、继发性牙骨质形成平均百分比（14.50% ± 6.65%）、成牙骨质细胞平均数量（10.25 ± 2.36）和成牙骨质破骨细胞平均数量（9.75 ± 4.71），与对照组相应值（分别为13.75% ± 4.34%、8.50% ± 2.88%、7.75 ± 1.25和6.50 ± 3.10）相比，虽有升高但差异无统计学意义（P > 0.05）。
注射i-PRF虽能使牙骨质吸收百分比、继发性牙骨质形成、成牙骨质细胞数量和成牙骨质破骨细胞数量有所增加，但差异不显著。
