Martínez J, Minguell J J
Exp Hematol. 1987 Mar;15(3):221-5.
An increase in triglyceride synthesis has been observed in cultures of human bone marrow fibroblasts after the cells reach confluence. The addition of hydrocortisone further enhances triglyceride synthesis. Conditioned medium from confluent cultures also stimulates adipogenesis, probably mediated by a factor released through a hydrocortisone-dependent process. Subcultures derived from confluent cultures grown in the presence of hydrocortisone show a decrease in replicative capacity, as measured by DNA synthesis. This effect may represent the onset of a more differentiated phenotype, which seems to correspond to a cell showing a buoyant density of 1.052 g/ml and a high rate of triglyceride synthesis.
在人骨髓成纤维细胞达到汇合后，其培养物中甘油三酯合成增加。添加氢化可的松可进一步增强甘油三酯合成。汇合培养物的条件培养基也能刺激脂肪生成，这可能是由通过氢化可的松依赖性过程释放的一种因子介导的。在氢化可的松存在下生长的汇合培养物传代培养物，其DNA合成量测量显示复制能力下降。这种效应可能代表更分化表型的开始，这似乎对应于一种漂浮密度为1.052 g/ml且甘油三酯合成速率高的细胞。
