FOXP3基因多态性对乙型肝炎病毒相关性肝细胞癌的影响：一项病例对照研究。
The effect of FOXP3 genetic polymorphisms on correlations with hepatitis B virus-hepatocellular carcinoma: A case-control study.
作者信息
Zhang Xiaolian, Li Jinwan, Qin Xue, Li Shan, Liang Dong
机构信息
Department of Clinical Laboratory, the First Affiliated Hospital of Guangxi Medical University, Key Laboratory of Clinical Laboratory Medicine of Guangxi Department of Education, Nanning, Guangxi, China.
School of Basic Medical Sciences, Guangxi Medical University, Nanning, Guangxi, China.
出版信息
Heliyon. 2023 Dec 12;10(1):e23660. doi: 10.1016/j.heliyon.2023.e23660. eCollection 2024 Jan 15.
BACKGROUND
Previous studies have reported that transcription factor forkhead box protein 3 (FOXP3) polymorphisms are correlated with the progress of some cancers, but the relationships between the FOXP3 polymorphisms and hepatocellular carcinoma (HCC) risk remain unclear.
METHOD
Genotypes were detected in156 hepatitis B virus (HBV)-HCC patients, 109 HBV-liver cirrhosis (LC) patients, 125 chronic hepatitis B (CHB) patients, and 188 healthy controls. The FOXP3 rs3761547 and rs3761548 polymorphisms were genotyped by polymerase chain reaction (PCR) combined with restriction fragment length polymorphism, and the rs2232365 polymorphism was genotyped using PCR with sequence-specific primers.
RESULTS
We did not obtain any significant results with the FOXP3 rs3761547, rs3761548, and rs2232365 polymorphisms in groups of patients compared to healthy controls (all > 0.05), no matter the overall group or subgroup.
CONCLUSIONS
Our findings suggest that the FOXP3 polymorphisms at rs3761547, rs3761548, and rs2232365 were not related to HBV-HCC risk in the Chinese population.
背景
既往研究报道转录因子叉头框蛋白3（FOXP3）基因多态性与某些癌症的进展相关，但FOXP3基因多态性与肝细胞癌（HCC）风险之间的关系仍不明确。
方法
对156例乙型肝炎病毒（HBV）相关HCC患者、109例HBV相关肝硬化（LC）患者、125例慢性乙型肝炎（CHB）患者及188例健康对照进行基因分型。采用聚合酶链反应（PCR）联合限制性片段长度多态性方法对FOXP3 rs3761547和rs3761548基因多态性进行基因分型，采用序列特异性引物PCR方法对rs2232365基因多态性进行基因分型。
结果
与健康对照相比，无论在总体组还是亚组中，FOXP3 rs3761547、rs3761548和rs2232365基因多态性在患者组中均未获得任何显著结果（均P>0.05）。
结论
我们的研究结果表明，rs3761547、rs3761548和rs2232365位点的FOXP3基因多态性与中国人群HBV-HCC风险无关。
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