Key Laboratory of Ecosystem Carbon Source and Sink, China Meteorological Administration (ECSS-CMA), School of Ecology and Applied Meteorology, Nanjing University of Information Science and Technology, Nanjing, 210044, China.
Appl Microbiol Biotechnol. 2024 Dec;108(1):47. doi: 10.1007/s00253-023-12876-8. Epub 2024 Jan 4.
Candidatus Methylomirabilis-related bacteria conduct anaerobic oxidation of methane (AOM) coupling with NO reduction, and Candidatus Methanoperedens-related archaea perform AOM coupling with reduction of diverse electron acceptors, including NO, Fe (III), Mn (IV) and SO. Application of nitrogen fertilization favors the growth of these methanotrophs in agricultural fields. Here, we explored the vertical variations in community structure and abundance of the two groups of methanotrophs in a nitrogen-rich vegetable field via using illumina MiSeq sequencing and quantitative PCR. The retrieved Methylomirabilis-related sequences had 91.12%-97.32% identity to the genomes of known Methylomirabilis species, and Methanoperedens-related sequences showed 85.49%-97.48% identity to the genomes of known Methanoperedens species which are capable of conducting AOM coupling with reduction of NO or Fe (III). The Methanoperedens-related archaeal diversity was significantly higher than Methylomirabilis-related bacteria, with totally 74 and 16 operational taxonomic units, respectively. In contrast, no significant difference in abundance between the bacteria (9.19 × 10-3.83 × 10 copies g dry soil) and the archaea (1.55 × 10-3.24 × 10 copies g dry soil) was observed. Furthermore, the abundance of both groups of methanotrophs exhibited a strong vertical variation, which peaked at 30-40 and 20-30 cm layers, respectively. Soil water content and pH were the key factors influencing Methylomirabilis-related bacterial diversity and abundance, respectively. For the Methanoperedens-related archaea, both soil pH and ammonium content contributed significantly to the changes of these archaeal diversity and abundance. Overall, we provide the first insights into the vertical distribution and regulation of Methylomirabilis-related bacteria and Methanoperedens-related archaea in vegetable soils. KEY POINTS: • The archaeal diversity was significantly higher than bacterial. • There was no significant difference in the abundance between bacteria and archaea. • The abundance of bacteria and archaea peaked at 30-40 and 20-30 cm, respectively.
与硝酸盐还原耦合的候选甲基厌氧甲烷氧化菌（Anaerobic Oxidation of Methane，AOM）和与多种电子受体（包括硝酸盐、三价铁、四价锰和硫酸盐）还原耦合的候选甲烷泥菌（Methanoperedens）进行 AOM。农业领域中施加氮肥有利于这些甲烷营养菌的生长。在这里，我们通过 illumina MiSeq 测序和定量 PCR 探索了富含氮的蔬菜田中这两组甲烷营养菌的垂直分布变化。与已知的甲基厌氧甲烷氧化菌（Methylomirabilis）种的基因组相比，回收的甲基厌氧甲烷氧化菌（Methylomirabilis）相关序列具有 91.12%-97.32%的同一性，与能够进行与硝酸盐或三价铁还原耦合的 AOM 的已知甲烷泥菌（Methanoperedens）种的基因组相比，Methanoperedens 相关序列显示 85.49%-97.48%的同一性。Methanoperedens 相关古菌的多样性明显高于甲基厌氧甲烷氧化菌（Methylomirabilis）相关细菌，分别有 74 和 16 个操作分类单位。相比之下，细菌（9.19×10-3.83×10 拷贝 g 干土）和古菌（1.55×10-3.24×10 拷贝 g 干土）的丰度没有显著差异。此外，两组甲烷营养菌的丰度都表现出强烈的垂直变化，分别在 30-40 和 20-30 cm 层达到峰值。土壤水分含量和 pH 值分别是影响甲基厌氧甲烷氧化菌（Methylomirabilis）相关细菌多样性和丰度的关键因素。对于 Methanoperedens 相关古菌，土壤 pH 值和铵含量都显著影响这些古菌多样性和丰度的变化。总的来说，我们首次提供了关于蔬菜土壤中甲基厌氧甲烷氧化菌（Methylomirabilis）相关细菌和 Methanoperedens 相关古菌的垂直分布和调控的见解。
