Pharmaceutical Sciences Division, University of Wisconsin-Madison, Madison, WI, USA.
Small Molecule Screening Facility, UW Carbone Cancer Center, Madison, WI, USA.
Microb Genom. 2024 Jan;10(1). doi: 10.1099/mgen.0.001167.
Investigations of the bacterial family have enabled the development of secondary metabolites critical to human health. Historical investigation of bacterial families for natural product discovery has focused on terrestrial strains, where time-consuming isolation processes often lead to the rediscovery of known compounds. To investigate the secondary metabolite potential of marine-derived , 38 strains were sequenced, assembled and analysed using antiSMASH and BiG-SLiCE. BiG-SLiCE contains a near-comprehensive dataset of approximately 1.2 million publicly available biosynthetic gene clusters from primarily terrestrial strains. Our marine-derived were directly compared to BiG-SLiCE’s preprocessed database using BiG-SLiCE’s query mode; genetic diversity within our strains was uncovered using BiG-SCAPE and metric multidimensional scaling analysis. Our analysis of marine-derived emphasizes the clear need for broader genomic investigations of marine strains to fully realize their potential as sources of new natural products.
对细菌家族的研究使对人类健康至关重要的次生代谢物得以发展。为了从天然产物中发现细菌家族，历史上的研究主要集中在陆地菌株上，而在陆地菌株中，耗时的分离过程往往导致已知化合物的重新发现。为了研究海洋来源的细菌的次生代谢物潜力，我们对 38 株细菌进行了测序、组装和分析，使用了 antiSMASH 和 BiG-SLiCE。BiG-SLiCE 包含了一个大约 120 万来自主要陆地菌株的公开生物合成基因簇的近乎完整的数据集。我们利用 BiG-SLiCE 的查询模式，直接将海洋来源的细菌与 BiG-SLiCE 的预处理数据库进行比较；利用 BiG-SCAPE 和度量多维尺度分析揭示了我们的菌株内的遗传多样性。我们对海洋来源的细菌的分析强调了对海洋菌株进行更广泛的基因组研究的明显需要，以充分实现它们作为新型天然产物来源的潜力。
