Ningbo Institute of Life and Health Industry, University of Chinese Academy of Sciences, Key Laboratory of Diagnosis and Treatment of Digestive System Tumors of Zhejiang Province, Ningbo No. 2 Hospital, Ningbo, 315012, China.
Beijing Key Laboratory of Microstructure and Properties of Solids, Faculty of Materials and Manufacturing, Beijing University of Technology, Beijing, 100124, China.
Adv Healthc Mater. 2024 Mar;13(7):e2301146. doi: 10.1002/adhm.202301146. Epub 2024 Jan 14.
Ribonucleic acids (RNA) play active roles within cells or viruses by catalyzing biological reactions, controlling gene expression, and communicating responses to cellular signals. Rapid monitoring RNA variation has become extremely important for appropriate clinical decisions and frontier biological research. However, the most widely used method for RNA detection, nucleic acid amplification, is restricted by a mandatory temperature cycling period of ≈1 h required to reach target detection criteria. Herein, a direct detection approach via single-atom site integrated surface-enhanced Raman scattering (SERS) monitoring nucleic acid pairing reaction, can be completed within 3 min and reaches high sensitivity and extreme reproducibility for COVID-19 and two other influenza viruses' detection. The mechanism is that a single-atom site on SERS chip, enabled by positioning a single-atom oxide coordinated with a specific complementary RNA probe on chip nanostructure hotspots, can effectively bind target RNA analytes to enrich them at designed sites so that the binding reaction can be detected through Raman signal variation. This ultrafast, sensitive, and reproducible single-atom site SERS chip approach paves the route for an alternative technique of immediate RNA detection. Moreover, single-atom site SERS is a novel surface enrichment strategy for SERS active sites for other analytes at ultralow concentrations.
核糖核酸（RNA）在细胞或病毒中通过催化生物反应、控制基因表达和对细胞信号做出反应来发挥积极作用。快速监测 RNA 变异对于做出适当的临床决策和前沿生物学研究变得极为重要。然而，最广泛用于 RNA 检测的方法——核酸扩增受到 ≈1 小时的强制性温度循环周期的限制，该周期是达到目标检测标准所必需的。在此，通过单原子位点集成的表面增强拉曼散射（SERS）监测核酸配对反应的直接检测方法可以在 3 分钟内完成，并实现了对 COVID-19 和另外两种流感病毒检测的高灵敏度和极高的重现性。其机制是，SERS 芯片上的单原子位点通过在芯片纳米结构热点上定位与特定互补 RNA 探针配位的单原子氧化物来实现，能够有效地将靶标 RNA 分析物结合以将其富集到设计的位点，从而可以通过拉曼信号变化检测到结合反应。这种超快速、灵敏且可重现的单原子位点 SERS 芯片方法为即时 RNA 检测开辟了替代技术的途径。此外，单原子位点 SERS 是用于其他超低浓度分析物的 SERS 活性位点的新型表面富集策略。
