Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin, 541004, China.
Mikrochim Acta. 2021 Apr 24;188(5):175. doi: 10.1007/s00604-021-04828-8.
Bisphenol A (BPA), as a typical endocrine disruptor, poses a serious threat to human health. Therefore, it is urgent to establish a rapid, sensitive, and simple method for the determination of BPA. In this paper, based on the aptamer-mediated single-atom Fe carbon dot catalyst (SA) catalyzing the HAuCl-ethylene glycol (EG) nanoreaction, a new SERS/RRS di-mode detection method for BPA was established. The results show that SA exhibits a strong catalytic effect on the HAuCl-EG nanoreaction, which could generate purple gold nanoparticles (AuNPs) with resonance Rayleigh scattering (RRS) signals and surface-enhanced Raman scattering (SERS) effects. After the addition of BPA aptamer (Apt), it could encapsulate SA through intermolecular interaction, thus inhibiting its catalytic action, resulting in the reduction of AuNPs generated and the decrease of RRS and SERS signals of the system. With the addition of BPA, Apt was specifically combined with BPA, and SA was re-released to restore the catalytic ability; the generated AuNPs increased. As a result of this RRS and SERS signals of the system recovered, and their increment was linear with the concentration of BPA. Thus, the quantification of 0.1-4.0 nM (RRS) and 0.1-12.0 nM (SERS) BPA was realized, and the detection limits were 0.08 nM and 0.03 nM, respectively. At the same time, we used molecular spectroscopy and electron microscopy to study the SA-HAuCl-ethylene glycol indicator reaction, and proposed a reasonable SA catalytic reaction mechanism. Based on Apt-mediated SA catalysis gold nanoreaction amplification, a SERS/RRS di-mode analytical platform was established for targets such as BPA.
双酚 A（BPA）作为一种典型的内分泌干扰物，对人类健康构成严重威胁。因此，建立一种快速、灵敏、简单的 BPA 测定方法迫在眉睫。本文基于适体介导的单原子 Fe 碳点催化剂（SA）催化 HAuCl-乙二醇（EG）纳米反应，建立了一种新的 BPA 的 SERS/RRS 双模检测方法。结果表明，SA 对 HAuCl-EG 纳米反应表现出很强的催化作用，可生成具有共振瑞利散射（RRS）信号和表面增强拉曼散射（SERS）效应的紫色金纳米粒子（AuNPs）。加入 BPA 适体（Apt）后，它可以通过分子间相互作用包裹 SA，从而抑制其催化作用，导致生成的 AuNPs 减少，体系的 RRS 和 SERS 信号降低。加入 BPA 后，Apt 特异性地与 BPA 结合，SA 被重新释放，恢复催化能力；生成的 AuNPs 增加。由于体系的 RRS 和 SERS 信号恢复，其增量与 BPA 的浓度呈线性关系。因此，实现了 0.1-4.0 nM（RRS）和 0.1-12.0 nM（SERS）BPA 的定量检测，检测限分别为 0.08 nM 和 0.03 nM。同时，我们利用分子光谱和电子显微镜研究了 SA-HAuCl-乙二醇指示剂反应，并提出了合理的 SA 催化反应机制。基于 Apt 介导的 SA 催化金纳米反应放大，建立了一种用于 BPA 等靶标的 SERS/RRS 双模分析平台。
