Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China; Key Laboratory for Natural Active Pharmaceutical Constituents Research in Universities of Shandong Province, School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.
Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China; College of pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250300, China.
Int J Biol Macromol. 2024 Feb;259(Pt 1):129128. doi: 10.1016/j.ijbiomac.2023.129128. Epub 2024 Jan 3.
Massa Medicata Fermentata (MMF) is a fermented food with therapeutic effects. Previous studies suggested that after stir-frying, the uronic acid content in MMF crude polysaccharides increases, and the pH value decreases, which is caused by the change in acidic polysaccharides. However, the detailed physicochemical properties and structure-activity correlation of the acidic polysaccharides in MMF have not been fully explored. In this study, two acidic polysaccharides (SMMFAP and CMMFAP) were isolated from the MMF and its stir-fried product, respectively. Their structural characteristics and bioactivities were comparatively studied, and the structure-activity correlation was examined. Our findings revealed that the SMMFAP had a higher average Mw and higher Gal and Man content than the CMMFAP. Both the SMMFAP and CMMFAP were mainly composed of Xyl, Man, and Gal residues, whereas the CMMFAP had fewer linkage types. Additionally, the CMMFAP exhibited stronger neuroprotective activity than the SMMFAP owing to its higher content of 1,6-linked-Galp, while the SMMFAP exhibited better antioxidant activity, which might be related to its higher average Mw. Our findings suggest that acidic polysaccharides may be the active substances that cause differences in effectiveness between the sheng and chao MMF. Furthermore, the research qualified the SMMFAP and CMMFAP with different potential applications.
发酵神曲（Massa Medicata Fermentata，MMF）是一种具有治疗作用的发酵食品。先前的研究表明，经过炒制后，MMF 粗多糖中的糖醛酸含量增加，pH 值降低，这是由于酸性多糖发生了变化。然而，MMF 中酸性多糖的详细理化性质和结构-活性关系尚未得到充分探索。本研究分别从 MMF 及其炒制产物中分离得到两种酸性多糖（SMMFAP 和 CMMFAP）。对其结构特征和生物活性进行了比较研究，并考察了结构-活性关系。研究结果表明，SMMFAP 的平均分子量（Mw）较高，Gal 和 Man 含量高于 CMMFAP。SMMFAP 和 CMMFAP 均主要由 Xyl、Man 和 Gal 残基组成，而 CMMFAP 的连接类型较少。此外，CMMFAP 由于含有较高比例的 1,6-连接-Galp，具有更强的神经保护活性，而 SMMFAP 则具有更好的抗氧化活性，这可能与其较高的平均 Mw 有关。研究结果表明，酸性多糖可能是造成生、炒 MMF 功效差异的活性物质。此外，本研究还为 SMMFAP 和 CMMFAP 赋予了不同的潜在应用价值。
