人红细胞谷丙转氨酶（GPT，酶编号2.6.1.2）的琼脂糖凝胶电泳。

Agarose gel electrophoresis of human erythrocyte glutamic-pyruvic transaminase (GPT EC 2.6.1.2).

作者信息

Bär W, Biedermann V

出版信息

Forensic Sci Int. 1987 Jan;33(1):69-73. doi: 10.1016/0379-0738(87)90141-1.

Abstract

The method of choice to determine erythrocyte glutamate-pyruvic transaminase (GPT) including rare variants was starch gel electrophoresis. Methods using agarose as gel medium were not reported to our knowledge. We present an adapted method using the Tris/maleate buffer system and an agarose of low endosmosis. The common as well as rare variant types of GPT were quickly and reliably separated. In addition, a method for the consecutive determination of esterase D (ESD) and GPT on the same gel using the malic acid buffer system is described.

摘要

用于测定包括稀有变体在内的红细胞谷丙转氨酶（GPT）的首选方法是淀粉凝胶电泳。据我们所知，尚未有使用琼脂糖作为凝胶介质的方法的相关报道。我们介绍一种采用Tris/马来酸盐缓冲系统和低渗琼脂糖的改良方法。常见以及稀有变体类型的GPT都能被快速且可靠地分离。此外，还描述了一种使用苹果酸缓冲系统在同一凝胶上连续测定酯酶D（ESD）和GPT的方法。