在含有隔板的超薄聚丙烯酰胺凝胶上进行非平衡pH梯度电泳（NEPHGE）：改进红细胞裂解物和血迹中红细胞磷酸葡萄糖变位酶（PGM）和酯酶D（EsD）的诊断。
Non-equilibrium pH gradient electrophoresis (NEPHGE) on ultrathin polyacrylamide gels containing separators: improved erythrocyte phosphoglucomutase (PGM) and esterase D (EsD) diagnosis in red cell lysates and bloodstains.
作者信息
Destro-Bisol G, Spinella A
机构信息
Istituto di Medicina Legale e delle Assicurazioni dell'Universitá Cattolica del Sacro Cuore, Italy.
出版信息
Forensic Sci Int. 1989 Jul;42(1-2):43-50. doi: 10.1016/0379-0738(89)90197-7.
PMID:2527190
Abstract
Two rapid and reliable electrophoretic techniques for PGM1 and EsD typing on ultrathin polyacrylamide gels are described. They have been based on non-equilibrium pH gradient electrophoresis and on the addition of chemical spacers (EPPS for PGM1 and HEPES for EsD) to the gel mixture.
摘要
本文描述了两种在超薄聚丙烯酰胺凝胶上对PGM1和EsD进行分型的快速可靠的电泳技术。它们基于非平衡pH梯度电泳，并在凝胶混合物中添加了化学间隔物（PGM1用EPPS，EsD用HEPES）。
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