Programa de Pós-Graduação em Biotecnologia em Saúde Humana e Animal da Universidade Estadual do Ceará, Fortaleza - Ceará, Brazil.
Departamento de Medicina Interna da Faculdade de Medicina da Universidade Federal do Ceará, Fortaleza - Ceará, Brazil.
Adv Rheumatol. 2024 Jan 4;64(1):6. doi: 10.1186/s42358-023-00346-8.
Despite some knowledge gaps in scientific evidence, MgCl is largely used for pain relief in musculoskeletal diseases. Mg salts were shown to provide analgesia postoperatively in orthopedic surgery and low Mg levels were linked to arthritis development and severity. We determined the anti-inflammatory activity of MgCl in an acute arthritis model.
Mice received 0.1 mg/25µL Zymosan (Zy) or saline into the knees. Joint pain was evaluated using von Frey test; cell influx, and interleukin (IL)-1 level were assessed in joint lavage at 6 h. Synovia were excised for histopathology and analysis of immunoexpression of nuclear factor kappa B (NFκB) and tumor necrosis factor (TNF)-α. Groups (n = 6/group) received either 90 mg/kg MgCl/100 µL or saline per os (systemic) or 500 µg/25 µL MgCl or saline intra-articularly (i.a.) 30 min prior to Zy.
MgCl given either systemically or locally significantly reduced cell influx (p = 0.0012 and p = 0.0269, respectively), pain (p = 0.0005 and p = 0.0038, respectively), and intra-articular IL-1 level (p = 0.0391), as compared to saline. Systemic MgCl significantly decreased NFκB (p < 0.05) immmunoexpression, as compared to saline.
MgCl given systemically or locally displayed anti-inflammatory activity in a severe acute arthritis model reducing cell influx, pain, and cytokine release. MgCl operates at least partially via inhibiting NFκB activation. This is the first in vivo demonstration that MgCl decreases cytokine release in arthritis, prompting reduction of inflammation and pain relief.
尽管在科学证据方面存在一些知识空白，但 MgCl 在肌肉骨骼疾病的疼痛缓解中被广泛应用。研究表明，Mg 盐在骨科手术后具有镇痛作用，低 Mg 水平与关节炎的发展和严重程度有关。我们在急性关节炎模型中确定了 MgCl 的抗炎活性。
小鼠膝关节内注射 0.1mg/25µL 酵母聚糖（Zy）或生理盐水。使用 von Frey 试验评估关节疼痛；在 6 小时时通过关节灌洗评估细胞流入和白细胞介素（IL）-1 水平。切除滑膜进行组织病理学检查，并分析核因子 kappa B（NFκB）和肿瘤坏死因子（TNF）-α的免疫表达。各组（n=6/组）在注射 Zy 前 30 分钟分别给予口服 90mg/kg MgCl/100µL 或生理盐水（全身）或关节内 500µg/25µL MgCl 或生理盐水。
与生理盐水相比，全身或局部给予 MgCl 均显著减少细胞流入（p=0.0012 和 p=0.0269）、疼痛（p=0.0005 和 p=0.0038）和关节内 IL-1 水平（p=0.0391）。与生理盐水相比，全身给予 MgCl 还显著降低了 NFκB 的免疫表达（p<0.05）。
MgCl 全身或局部给药在严重急性关节炎模型中显示出抗炎活性，可减少细胞流入、疼痛和细胞因子释放。MgCl 至少部分通过抑制 NFκB 激活发挥作用。这是首次在体内证明 MgCl 可减少关节炎中的细胞因子释放，从而减轻炎症和缓解疼痛。
